Human Xeroderma Pigmentosum, Complementation Group C (XPC) ELISA Kit | XPC elisa kit

Human Xeroderma Pigmentosum, Complementation Group C (XPC) ELISA Kit

Cat. Num. AAA450052

• Gene Names: XPC; XP3; RAD4; XPCC; p125
• Reactivity: Human
• Synonyms: Xeroderma Pigmentosum; Complementation Group C (XPC); Human Xeroderma Pigmentosum; Complementation Group C (XPC) ELISA Kit; CCS; Copper Chaperone For Superoxide Dismutase; XPC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of XPC. No significant cross-reactivity or interference between XPC and analogues was observed.
• Sequence Length: 940

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.115ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level XPC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level XPC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for XPC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of XPC in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to XPC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to XPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain XPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of XPC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224809295
• NCBI GeneID: 7508
• NCBI Accession #: NP_004619.3
• NCBI GenBank Nucleotide #: NM_004628.4
• UniProt Accession #: Q01831; Q53GT7; Q96AX0; B4DIP3; E9PB96; E9PH69
• Molecular Weight: 15,712 Da
• NCBI Official Full Name: DNA repair protein complementing XP-C cells
• NCBI Official Synonym Full Names: XPC complex subunit, DNA damage recognition and repair factor
• NCBI Official Symbol: XPC
• NCBI Official Synonym Symbols: XP3; RAD4; XPCC; p125
• NCBI Protein Information: DNA repair protein complementing XP-C cells
• UniProt Protein Name: DNA repair protein complementing XP-C cells
• Protein Family: DNA repair protein
• UniProt Gene Name: XPC
• UniProt Synonym Gene Names: XPCC

NCBI Description

This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.