Typical Testing Data/Standard Curve (for reference only)
Human Chondrolectin (CHODL) ELISA Kit | CHODL elisa kit
Human Chondrolectin (CHODL) ELISA Kit
Gene Names
Chodl; MT75; PRED12; 3110074E07Rik
Reactivity
Human
Synonyms
Chondrolectin (CHODL); Human Chondrolectin (CHODL) ELISA Kit; CHODL elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CHODL. No significant cross-reactivity or interference between CHODL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHODL and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CHODL elisa kit
Intended Uses: This CHODL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CHODL. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CHODL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CHODL antibody and an CHODL-HRP conjugate. The assay sample and buffer are incubated together with CHODL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme- substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHODL concentration since CHODL from samples and CHODL-HRP conjugate compete for the anti-CHODL antibody binding site. Since the number of sites is limited, as more sites are occupied by CHODL from the sample, fewer sites are left to bind CHODL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHODL concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CHODL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CHODL antibody and an CHODL-HRP conjugate. The assay sample and buffer are incubated together with CHODL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme- substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHODL concentration since CHODL from samples and CHODL-HRP conjugate compete for the anti-CHODL antibody binding site. Since the number of sites is limited, as more sites are occupied by CHODL from the sample, fewer sites are left to bind CHODL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHODL concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,331 Da
NCBI Official Full Name
chondrolectin
NCBI Official Synonym Full Names
chondrolectin
NCBI Official Symbol
Chodl
NCBI Official Synonym Symbols
MT75; PRED12; 3110074E07Rik
NCBI Protein Information
chondrolectin; transmembrane protein MT75
UniProt Protein Name
Chondrolectin
Protein Family
UniProt Gene Name
Chodl
UniProt Entry Name
CHODL_MOUSE
Uniprot Description
CHODL: a type I membrane protein with a carbohydrate recognition domain characteristic of C-type lectins in its extracellular portion. In other proteins, this domain is involved in endocytosis of glycoproteins and exogenous sugar-bearing pathogens. This protein localizes predominantly to the perinuclear region. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Protein type: Membrane protein, integral; Endoplasmic reticulum
Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; integral to membrane
Molecular Function: hyaluronic acid binding; carbohydrate binding
Research Articles on CHODL
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Product Notes
The Human CHODL chodl (Catalog #AAA7220526) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7220526 ELISA Kit recognizes Human CHODL. It is sometimes possible for the material contained within the vial of "Chondrolectin (CHODL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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