Rabbit anti-Human DGKA Polyclonal Antibody | anti-DGKA antibody

DGKA, CT (Diacylglycerol Kinase alpha, Diglyceride Kinase alpha, DGK-alpha, DAG Kinase alpha, 80kD Diacylglycerol Kinase, DAGK, DAGK1) (APC)

Cat. Num. AAA6472384

• Gene Names: DGKA; DAGK; DAGK1; DGK-alpha
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DGKA; Polyclonal Antibody; CT (Diacylglycerol Kinase alpha; Diglyceride Kinase alpha; DGK-alpha; DAG Kinase alpha; 80kD Diacylglycerol Kinase; DAGK; DAGK1) (APC); anti-DGKA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DGKA.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 735

• Applicable Applications for anti-DGKA antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human DGKA (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-DGKA Antibody (C-term) in HL60 cell line lysates (35ug/lane). DGKA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-DGKA antibody

Antibodies; Protein Kinases

References

1. Hart, T.C., et al., Genomics 22(1):246-247 (1994). 2. Hart, T.C., et al., Mamm. Genome 5(2):123-124 (1994). 3. Schaap, D., et al., FEBS Lett. 275(1-2), 151-158 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 41872494
• NCBI GeneID: 1606
• NCBI Accession #: NP_958853
• NCBI GenBank Nucleotide #: NP_958853.1
• UniProt Accession #: P23743
• Molecular Weight: 82.672
• NCBI Official Full Name: diacylglycerol kinase alpha isoform a
• NCBI Official Synonym Full Names: diacylglycerol kinase alpha
• NCBI Official Symbol: DGKA
• NCBI Official Synonym Symbols: DAGK; DAGK1; DGK-alpha
• NCBI Protein Information: diacylglycerol kinase alpha
• UniProt Protein Name: Diacylglycerol kinase alpha
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKA
• UniProt Synonym Gene Names: DAGK; DAGK1; DAG kinase alpha; DGK-alpha
• UniProt Entry Name: DGKA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]

Uniprot Description

DGKA: Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. Monomer. Lymphocytes and oligodendroglial cells. Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; EC 2.7.1.107; Motility/polarity/chemotaxis; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: membrane; plasma membrane; cytosol

Molecular Function: phospholipid binding; calcium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation