Human VACM-1 (Cullin 5) ELISA Kit | VACM-1 elisa kit

Human VACM-1 (Cullin 5) ELISA Kit

Cat. Num. AAA7606107

• Gene Names: CUL5; CUL-5; VACM1; VACM-1
• Reactivity: Human
• Synonyms: VACM-1 (Cullin 5); Human VACM-1 (Cullin 5) ELISA Kit; VACM-1 elisa kit

• Reactivity: Human
• Sequence Length: 780

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VACM-1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- VACM-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-VACM-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VACM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VACM-1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 14917099
• NCBI GeneID: 8065
• UniProt Accession #: Q93034; O14766; Q9BZC6; A8K960
• Molecular Weight: 90,955 Da
• NCBI Official Full Name: Cullin-5
• NCBI Official Synonym Full Names: cullin 5
• NCBI Official Symbol: CUL5
• NCBI Official Synonym Symbols: CUL-5; VACM1; VACM-1
• NCBI Protein Information: cullin-5
• UniProt Protein Name: Cullin-5
• UniProt Gene Name: CUL5
• UniProt Synonym Gene Names: VACM1; CUL-5; VACM-1

Uniprot Description

CUL5: a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to be involved proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Member of the cullin family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Cul5-RING ubiquitin ligase complex; cytosol

Molecular Function: calcium channel activity; protein binding; receptor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: cell cycle arrest; cell proliferation; G1/S transition of mitotic cell cycle; negative regulation of cell proliferation; protein ubiquitination during ubiquitin-dependent protein catabolic process; response to DNA damage stimulus