Rabbit anti-Human AP3B2 Polyclonal Antibody | anti-AP3B2 antibody

AP3B2, NT (AP3B2, AP-3 complex subunit beta-2, Adapter-related protein complex 3 subunit beta-2, Adaptor protein complex AP-3 subunit beta-2, Beta-3B-adaptin, Clathrin assembly protein complex 3 beta-2 large chain, Neuron-specific vesicle coat protein bet

Cat. Num. AAA6274107

• Gene Names: AP3B2; NAPTB
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AP3B2; Polyclonal Antibody; NT (AP3B2; AP-3 complex subunit beta-2; Adapter-related protein complex 3 subunit beta-2; Adaptor protein complex AP-3 subunit beta-2; Beta-3B-adaptin; Clathrin assembly protein complex 3 beta-2 large chain; Neuron-specific vesicle coat protein bet; Neuron-specific vesicle coat protein beta-NAP; anti-AP3B2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AP3B2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AP3B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human AP3B2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AP3B2 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AP3B2 antibody detected the AP3B2 protein (arrow).)

Related Product Information for anti-AP3B2 antibody

Adaptor protein-3 (AP3) is a heterotetrameric vesicle-coat protein complex. Some AP3 subunits are ubiquitously expressed, whereas others are expressed exclusively in neurons. The neuron-specific AP3 complex, which includes AP3B2, is thought to serve neuron-specific functions such as neurotransmitter release (Grabner et al., 2006 [PubMed 16788073]).

Product Categories/Family for anti-AP3B2 antibody

Antibodies; Clathrin

References

Nicolas, E., et al. Eur. J. Hum. Genet. 18(10):1107-1113(2010). Hashimoto, R., et al. Neurosci. Res. 65(1):113-115(2009). Chen, C., et al. DNA Seq. 18(3):165-168(2007). Grabner, C.P., et al. Proc. Natl. Acad. Sci. U.S.A. 103(26):10035-10040(2006). Dubois, T., et al. Biochem. Biophys. Res. Commun. 301(2):502-508(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 34482047
• NCBI GeneID: 8120
• NCBI Accession #: NP_004635.2
• NCBI GenBank Nucleotide #: NM_004644.4
• UniProt Accession #: Q13367; O14808; Q52LY8; A4Z4T7; B7ZKR7; B7ZKS0
• Molecular Weight: 121,189 Da
• NCBI Official Full Name: AP-3 complex subunit beta-2 isoform 2
• NCBI Official Synonym Full Names: adaptor-related protein complex 3, beta 2 subunit
• NCBI Official Symbol: AP3B2
• NCBI Official Synonym Symbols: NAPTB
• NCBI Protein Information: AP-3 complex subunit beta-2; beta-3B-adaptin; Neuronal adaptin-like protein, beta-subunit; adaptor protein complex AP-3 subunit beta-2; neuron-specific vesicle coat protein beta-NAP; adapter-related protein complex 3 subunit beta-2; adaptor-related protei
• UniProt Protein Name: AP-3 complex subunit beta-2
• Protein Family: AP-3 complex
• UniProt Gene Name: AP3B2
• UniProt Entry Name: AP3B2_HUMAN

NCBI Description

Adaptor protein-3 (AP3) is a heterotetrameric vesicle-coat protein complex. Some AP3 subunits are ubiquitously expressed, whereas others are expressed exclusively in neurons. The neuron-specific AP3 complex, which includes AP3B2, is thought to serve neuron-specific functions such as neurotransmitter release (Grabner et al., 2006 [PubMed 16788073]).[supplied by OMIM, Mar 2008]

Uniprot Description

AP3B2: Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. Belongs to the adaptor complexes large subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 15q

Cellular Component: nucleoplasm; Golgi apparatus; COPI-coated vesicle; cytoplasm; clathrin coated vesicle membrane

Molecular Function: transporter activity

Biological Process: intracellular protein transport; anterograde synaptic vesicle transport; post-Golgi vesicle-mediated transport; anterograde axon cargo transport