Typical Testing Data/Standard Curve (for reference only)
Human V type proton ATPase subunit d 2 (ATP6V0D2) ELISA Kit | ATP6V0D2 elisa kit
Human V type proton ATPase subunit d 2 (ATP6V0D2) ELISA Kit
Gene Names
ATP6V0D2; VMA6; ATP6D2
Reactivity
Human
Synonyms
V type proton ATPase subunit d 2 (ATP6V0D2); Human V type proton ATPase subunit d 2 (ATP6V0D2) ELISA Kit; ATP6V0D2 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ATP6V0D2 elisa kit
Intended Uses||This ATP6V0D2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATP6V0D2. This ELISA kit for research use only!
Principle of the Assay: ATP6V0D2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP6V0D2 antibody and an ATP6V0D2-HRP conjugate. The assay sample and buffer are incubated together with ATP6V0D2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theATP6V0D2 concentration since ATP6V0D2 from samples and ATP6V0D2-HRP conjugate compete for the anti-ATP6V0D2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP6V0D2 from the sample, fewer sites are left to bind ATP6V0D2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP6V0D2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ATP6V0D2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP6V0D2 antibody and an ATP6V0D2-HRP conjugate. The assay sample and buffer are incubated together with ATP6V0D2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theATP6V0D2 concentration since ATP6V0D2 from samples and ATP6V0D2-HRP conjugate compete for the anti-ATP6V0D2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP6V0D2 from the sample, fewer sites are left to bind ATP6V0D2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP6V0D2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ATP6V0D2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,426 Da
NCBI Official Full Name
V-type proton ATPase subunit d 2
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2
NCBI Official Symbol
ATP6V0D2
NCBI Official Synonym Symbols
VMA6; ATP6D2
NCBI Protein Information
V-type proton ATPase subunit d 2; V-ATPase subunit d 2; vacuolar proton pump subunit d 2
UniProt Protein Name
V-type proton ATPase subunit d 2
Protein Family
UniProt Gene Name
ATP6V0D2
UniProt Synonym Gene Names
V-ATPase subunit d 2
UniProt Entry Name
VA0D2_HUMAN
Uniprot Description
ATP6V0D2: Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. Belongs to the V-ATPase V0D/AC39 subunit family.
Protein type: Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14
Chromosomal Location of Human Ortholog: -
Cellular Component: phagocytic vesicle membrane; membrane; apical plasma membrane; early endosome; endosome membrane; vacuolar proton-transporting V-type ATPase complex
Molecular Function: protein binding; hydrogen ion transmembrane transporter activity
Biological Process: interaction with host; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport
Research Articles on ATP6V0D2
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Product Notes
The Human ATP6V0D2 atp6v0d2 (Catalog #AAA7232235) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7232235 ELISA Kit recognizes Human ATP6V0D2. It is sometimes possible for the material contained within the vial of "V type proton ATPase subunit d 2 (ATP6V0D2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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