Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-conjugating enzyme E2 C (UBE2C) Standard Curve.)
Human Ubiquitin-Conjugating Enzyme E2 C (UBE2C) ELISA Kit | UBE2C elisa kit
Human Ubiquitin-Conjugating Enzyme E2 C (UBE2C) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-conjugating enzyme E2 C (UBE2C) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-conjugating enzyme E2 C (UBE2C) Standard Curve.)
Background: UBE2CBP bound UBE2C and that the amino acid sequence between 235 and 257 was necessary for this binding. UBE2CBP showed a self-ubiquitinylation activity in a HECT-like sequence-dependent manner. The C-terminal half (amino acids 188-389) showed stronger activity than the full-length UBE2CBP. In addition, the C-terminal half of UBE2CBP was able to bind cyclin B and ubiquitinylate cyclin B in vitro. UBE2CBP gene contains 10 exons and spans approximately 176 kb.The C terminus of UBE2CBP shows weak homology to the HECT domain, which is conserved in one of the ubiquitin ligase (E3) families. Members of this family accept the ubiquitin moiety from E2 and transfer it to the target protein.
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]
Uniprot Description
UBE2C: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ubiquitin conjugating system; Ligase; Cell cycle regulation; EC 6.3.2.19; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: nucleoplasm; anaphase-promoting complex; cytoplasm; cytosol
Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; protein ubiquitination; cyclin catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; exit from mitosis; positive regulation of exit from mitosis
Research Articles on UBE2C
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Product Notes
The Human UBE2C ube2c (Catalog #AAA9713770) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713770 ELISA Kit recognizes Human UBE2C. It is sometimes possible for the material contained within the vial of "Ubiquitin-Conjugating Enzyme E2 C (UBE2C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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