Human Ubiquilin 2 (UBQLN2) ELISA Kit | UBQLN2 elisa kit

Human Ubiquilin 2 (UBQLN2) ELISA Kit

Cat. Num. AAA2024092

• Gene Names: UBQLN2; DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157
• Reactivity: Human
• Synonyms: Ubiquilin 2 (UBQLN2); Human Ubiquilin 2 (UBQLN2) ELISA Kit; CHAP1; CHAP1/DSK2; Dsk2; LIC-2; N4BP4; PLIC-2; PLIC2; NEDD4 Binding Protein 4; Protein linking IAP with cytoskeleton 2; Ubiquitin-like product Chap1/Dsk2; UBQLN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquilin 2 (UBQLN2).; No significant cross-reactivity or interference between Ubiquilin 2 (UBQLN2) and analogues was observed.
• Sequence Length: 624

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.64ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquilin 2 (UBQLN2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquilin 2 (UBQLN2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UBQLN2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquilin 2 (UBQLN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquilin 2 (UBQLN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquilin 2 (UBQLN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquilin 2 (UBQLN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for UBQLN2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 119613639
• NCBI GeneID: 29978
• UniProt Accession #: Q9UHD9; O94798; Q5D027; Q9H3W6; Q9HAZ4
• Molecular Weight: 65,696 Da
• NCBI Official Full Name: ubiquilin 2
• NCBI Official Synonym Full Names: ubiquilin 2
• NCBI Official Symbol: UBQLN2
• NCBI Official Synonym Symbols: DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157
• NCBI Protein Information: ubiquilin-2
• UniProt Protein Name: Ubiquilin-2
• Protein Family: Ubiquilin
• UniProt Gene Name: UBQLN2
• UniProt Synonym Gene Names: N4BP4; PLIC2; PLIC-2; hPLIC-2
• UniProt Entry Name: UBQL2_HUMAN

NCBI Description

This gene encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases; and thus, are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein. [provided by RefSeq, Oct 2009]

Uniprot Description

UBQLN2: Increases the half-life of proteins destined to be degraded by the proteasome; may modulate proteasome-mediated protein degradation. Defects in UBQLN2 are the cause of amyotrophic lateral sclerosis type 15 with or without frontotemporal dementia (ALS15). A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. Patients with ALS15 may develop frontotemporal dementia.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding

Disease: Amyotrophic Lateral Sclerosis 15, With Or Without Frontotemporal Dementia