Human Tumor Protein p53 Binding Protein 1 (TP53BP1) ELISA Kit | TP53BP1 elisa kit

Human Tumor Protein p53 Binding Protein 1 (TP53BP1) ELISA Kit

Cat. Num. AAA2023431

• Gene Names: TP53BP1; p202; 53BP1; p53BP1
• Reactivity: Human
• Synonyms: Tumor Protein p53 Binding Protein 1 (TP53BP1); Human Tumor Protein p53 Binding Protein 1 (TP53BP1) ELISA Kit; 53BP1; p53BP1; p202; TP53BP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Protein p53 Binding Protein 1 (TP53BP1).; No significant cross-reactivity or interference between Tumor Protein p53 Binding Protein 1 (TP53BP1) and analogues was observed.
• Sequence Length: 1,975

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.127ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein p53 Binding Protein 1 (TP53BP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein p53 Binding Protein 1 (TP53BP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TP53BP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein p53 Binding Protein 1 (TP53BP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Protein p53 Binding Protein 1 (TP53BP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein p53 Binding Protein 1 (TP53BP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Protein p53 Binding Protein 1 (TP53BP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TP53BP1 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 85566841
• NCBI GeneID: 7158
• UniProt Accession #: Q12888; Q2M1Z7; Q4LE46; Q5FWZ3; Q7Z3U4; F8VY86
• Molecular Weight: 213,989 Da
• NCBI Official Full Name: Tumor protein p53 binding protein 1
• NCBI Official Synonym Full Names: tumor protein p53 binding protein 1
• NCBI Official Symbol: TP53BP1
• NCBI Official Synonym Symbols: p202; 53BP1; p53BP1
• NCBI Protein Information: tumor suppressor p53-binding protein 1
• UniProt Protein Name: Tumor suppressor p53-binding protein 1
• Protein Family: Tumor suppressor p53-binding protein
• UniProt Gene Name: TP53BP1
• UniProt Synonym Gene Names: 53BP1; p53-binding protein 1; p53BP1
• UniProt Entry Name: TP53B_HUMAN

Uniprot Description

53BP1: a DNA damage checkpoint protein that interacts with HDAC4 protein to mediate the DNA damage response. Inhibits E2F1-mediated apoptosis in prostate cancer cells. Recruited to double strand breaks via the binding of its Tudor domain to dimethylated H3 K20. Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. Binds to the central domain of p53. Interacts with Artemis. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on S139. Interacts with histone H4 that has been dimethylated at K20. Has low affinity for histone H4 containing monomethylated K20. Does not bind histone H4 containing unmethylated or trimethylated K20. Has low affinity for histone H3 that has been dimethylated on K79. Does not bind unmethylated histone H3. Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Two alternatively spliced human isoforms have been observed.

Protein type: DNA repair, damage; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q15-q21

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasm; replication fork; nucleus

Molecular Function: protein binding; p53 binding; telomeric DNA binding; damaged DNA binding; methylated histone residue binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; double-strand break repair; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination