Carbonic Anhydrase VB Active Protein | CA5B active protein

Carbonic Anhydrase VB Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121145

• Gene Names: CA5B; CA-VB
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase VB; Carbonic Anhydrase VB Protein; Human; Recombinant (His Tag); Human Carbonic Anhydrase VB/CA5B Protein (His Tag); CA-VB Protein; MGC39962 Protein; carbonic anhydrase VB; mitochondrial; CA5B active protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 50mM NaCl, 0.05% Brij-35, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Cys34-Pro317

• Species: Human
• Activity: Measured by its esterase activity. The specific activity is >150pmoles/min/ug.
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of human CA5B (Q9Y2D0)(Cys 34-Pro 317) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CA5B active protein

Background: Carbonic anhydrase 5B, also known as carbonate dehydratase VB, carbonic anhydrase VB, CA-VB and CA5B, is amember of the alpha-carbonic anhydrase family. The strongest expression of CA5B/CA-VB is in heart, pancreas, kidney, placenta, lung, and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B/CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A/CA-VA. CA5B/CA-VB has a wider tissue distribution than CA5A/CA-VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A/CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B/CA-VB is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin and Foscarnet (phosphonoformate trisodium salt).

References

Fujikawa-Adachi K, et al.,1999, J Biol Chem. 274 (30): 21228-33. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262. Temperini C.et al., 2006, Chemistry 12: 7057-66. Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222. Maresca, A.et al., 2009, J. Am. Chem. Soc. 131:3057-3062.

NCBI and Uniprot Product Information

• NCBI GI #: 6005723
• NCBI GeneID: 11238
• NCBI Accession #: NP_009151.1
• NCBI GenBank Nucleotide #: NM_007220.3
• UniProt Accession #: Q9Y2D0; A8K4T5
• Molecular Weight: 36,434 Da
• NCBI Official Full Name: carbonic anhydrase 5B, mitochondrial
• NCBI Official Synonym Full Names: carbonic anhydrase VB, mitochondrial
• NCBI Official Symbol: CA5B
• NCBI Official Synonym Symbols: CA-VB
• NCBI Protein Information: carbonic anhydrase 5B, mitochondrial; carbonic dehydratase; carbonate dehydratase VB
• UniProt Protein Name: Carbonic anhydrase 5B, mitochondrial
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA5B
• UniProt Synonym Gene Names: CA-VB
• UniProt Entry Name: CAH5B_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. [provided by RefSeq, Jul 2008]

Uniprot Description

CA5B: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1; Mitochondrial

Chromosomal Location of Human Ortholog: Xp21.1

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process