Human tumor necrosis factor-related apoptosis-inducing ligand 3, TRAIL-R3 ELISA Kit | TRAIL-R3 elisa kit

Human tumor necrosis factor-related apoptosis-inducing ligand receptor 3, TRAIL-R3 ELISA Kit

Cat. Num. AAA700949

• Reactivity: Human
• Synonyms: tumor necrosis factor-related apoptosis-inducing ligand 3; TRAIL-R3; Human tumor necrosis factor-related apoptosis-inducing ligand receptor 3; TRAIL-R3 ELISA Kit; TRAIL-R3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TRAIL-R3. No significant cross-reactivity or interference between Human TRAIL-R3 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRAIL-R3 elisa kit

Introduction: TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. TRAIL binds at least five different receptors: TRAIL R1 (DR4), R2 (DR5), R3 (DcR1), R4 (DcR2), and osteoprotegerin (OPG). TRAIL receptors all exhibit cysteine-rich repeats characteristic of the TNF receptor superfamily. TRAIL R1 and R2 are type 1 transmembrane proteins with intracellular death domains (DD) and, subsequently, have the ability to mediate TRAIL-induced apoptosis. The remaining receptors are decoys that may regulate TRAIL activity by competing for binding sites with TRAIL R1 and R2. TRAIL R3 is membrane-associated via GPI linkage. TRAIL R4 is a type 1 transmembrane receptor, but exhibits a truncated cytoplasmic domain. Like the active receptors TRAIL R1 and R2, OPG has a DD motif but is a soluble protein.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRAIL-R3. Standards or samples are then added to the appropriate microtiter plate wells. And a HRP-conjugated antibody is added to each microplate well and incubated. Then substrate solution is added to each well. Only those wells that contain TRAIL-R3, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TRAIL-R3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.