Human tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain ELISA Kit | TNFRSF10D elisa kit

Human tumor necrosis factor-related apoptosis-inducing ligand receptor 4, TRAIL-R4 ELISA Kit

Cat. Num. AAA700345

• Gene Names: TNFRSF10D; DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4
• Reactivity: Human
• Synonyms: tumor necrosis factor receptor superfamily; member 10d; decoy with truncated death domain; Human tumor necrosis factor-related apoptosis-inducing ligand receptor 4; TRAIL-R4 ELISA Kit; Human tumor necrosis factor-related apoptosis-inducing ligand receptor 4 (TRAIL-R4) ELISA Kit; CD264; DCR2; TRAILR4; TRUNDD; TNF receptor-related receptor for TRAIL; TRAIL receptor 4; TRAIL receptor with a truncated death domain; decoy receptor 2; tumor necrosis; TNFRSF10D elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TNFRSF10D. No significant cross-reactivity or interference between Human TNFRSF10D and analogues was observed.
• Sequence Length: 386

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apoptosis/Autophagy
• Protein Biological Process 3: Apoptosis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFRSF10D elisa kit

Introduction: TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. TRAIL binds at least five different receptors: TRAIL R1 (DR4), R2 (DR5), R3 (DcR1), R4 (DcR2), and osteoprotegerin (OPG). TRAIL receptors all exhibit cysteine-rich repeats characteristic of the TNF receptor superfamily. TRAIL R1 and R2 are type 1 transmembrane proteins with intracellular death domains (DD) and, subsequently, have the ability to mediate TRAIL-induced apoptosis. The remaining receptors are decoys that may regulate TRAIL activity by competing for binding sites with TRAIL R1 and R2. TRAIL R3 is membrane-associated via GPI linkage. TRAIL R4 is a type 1 transmembrane receptor, but exhibits a truncated cytoplasmic domain. Like the active receptors TRAIL R1 and R2, OPG has a DD motif but is a soluble protein.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRAIL-R4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TRAIL-R4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TRAIL-R4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TRAIL-R4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22547108
• NCBI GeneID: 8793
• NCBI Accession #: NP_003831.2
• NCBI GenBank Nucleotide #: NM_003840.4
• UniProt Accession #: Q9UBN6; Q9Y6Q4; B2R8W0
• Molecular Weight: 41,823 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 10D
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
• NCBI Official Symbol: TNFRSF10D
• NCBI Official Synonym Symbols: DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10D; TRAIL receptor 4; decoy receptor 2; TNF receptor-related receptor for TRAIL; TRAIL receptor with a truncated death domain; TNF-related apoptosis-inducing ligand receptor 4
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 10D
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF10D
• UniProt Synonym Gene Names: DCR2; TRAILR4; TRUNDD; UNQ251/PRO288; DcR2; TRAIL receptor 4; TRAIL-R4
• UniProt Entry Name: TR10D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to 1 PublicationRef.1