Human Tumor Necrosis Factor-Like Weak Inducer Of Apoptosis (TWEAK) ELISA Kit | TWEAK elisa kit

Human Tumor Necrosis Factor-Like Weak Inducer Of Apoptosis (TWEAK) ELISA Kit

Cat. Num. AAA3801765

• Gene Names: TNFSF12; APO3L; DR3LG; TWEAK; TNLG4A
• Reactivity: Human
• Synonyms: Tumor Necrosis Factor-Like Weak Inducer Of Apoptosis (TWEAK); Human Tumor Necrosis Factor-Like Weak Inducer Of Apoptosis (TWEAK) ELISA Kit; TWEAK elisa kit

• Reactivity: Human
• Sequence Length: 249

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 100 pg/ml-1600 pg/ml
• Sensitivity: 10 pg/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TWEAK elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofTWEAKin the sample, This TWEAKELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusTWEAKconcentration. The concentration ofTWEAKin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507597
• NCBI GeneID: 8742
• NCBI Accession #: NP_003800.1
• NCBI GenBank Nucleotide #: NP_003800.1
• UniProt Accession #: O43508
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 12 proprotein
• NCBI Official Synonym Full Names: TNF superfamily member 12
• NCBI Official Symbol: TNFSF12
• NCBI Official Synonym Symbols: APO3L; DR3LG; TWEAK; TNLG4A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 12
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 12
• UniProt Gene Name: TNFSF12
• UniProt Synonym Gene Names: APO3L; DR3LG; TWEAK
• UniProt Entry Name: TNF12_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q. Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. Belongs to the tumor necrosis factor family.

Protein type: Motility/polarity/chemotaxis; Apoptosis; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; integral to plasma membrane; perinuclear region of cytoplasm

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: positive regulation of angiogenesis; apoptosis; positive regulation of protein catabolic process; endothelial cell migration; immune response; positive regulation of endothelial cell proliferation; angiogenesis; cell differentiation; signal transduction