Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Scavenger receptor class A member 5 (SCARA5) Standard Curve.)
Human Scavenger Receptor Class A Member 5 (SCARA5) ELISA Kit | SCARA5 elisa kit
Human Scavenger Receptor Class A Member 5 (SCARA5) ELISA Kit
Gene Names
SCARA5; Tesr; NET33
Reactivity
Human
Synonyms
Scavenger Receptor Class A Member 5 (SCARA5); Human Scavenger Receptor Class A Member 5 (SCARA5) ELISA Kit; SCARA5; FLJ23907; MGC45780; NET33; Tesr; scavenger receptor class A; member 5; testis expressed scavenger receptor; SCARA5 elisa kit
Reactivity
Human
Specificity
Human Scavenger receptor class A member 5 (SCARA5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SCARA5. No significant cross-reactivity or interference between Human SCARA5 and analogues was observed.
Sequence Length
495
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Scavenger receptor class A member 5 (SCARA5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Scavenger receptor class A member 5 (SCARA5) Standard Curve.)
Related Product Information for SCARA5 elisa kit
Description: This Human Scavenger receptor class A member 5 (SCARA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SCARA5 in samples. An antibody specific for SCARA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCARA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCARA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCARA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Scara5, encoding a deduced 491-amino acid protein that shares 88% sequence identity with the 495-amino acid human ortholog. Although overall identity with Scara1 is about 25%, Scara5 and Scara1 share many features, including an N-terminal cytoplasmic domain followed by a predicted transmembrane domain, a spacer region with several putative glycosylation sites, a collagenous domain, a hinge region, and a C-terminal scavenger receptor cysteine-rich domain (SRCR). The SCARA5 protein was predicted to be trimeric, as are other collagenous transmembrane proteins, and this prediction was supported by Western blotting. Based on similarity to other members of this family, the trimer is present on the surface of the plasma membrane and anchored by the transmembrane domain.
Background: Scara5, encoding a deduced 491-amino acid protein that shares 88% sequence identity with the 495-amino acid human ortholog. Although overall identity with Scara1 is about 25%, Scara5 and Scara1 share many features, including an N-terminal cytoplasmic domain followed by a predicted transmembrane domain, a spacer region with several putative glycosylation sites, a collagenous domain, a hinge region, and a C-terminal scavenger receptor cysteine-rich domain (SRCR). The SCARA5 protein was predicted to be trimeric, as are other collagenous transmembrane proteins, and this prediction was supported by Western blotting. Based on similarity to other members of this family, the trimer is present on the surface of the plasma membrane and anchored by the transmembrane domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
scavenger receptor class A member 5
NCBI Official Synonym Full Names
scavenger receptor class A member 5
NCBI Official Symbol
SCARA5
NCBI Official Synonym Symbols
Tesr; NET33
NCBI Protein Information
scavenger receptor class A member 5
UniProt Protein Name
Scavenger receptor class A member 5
Protein Family
UniProt Gene Name
SCARA5
UniProt Entry Name
SCAR5_HUMAN
Uniprot Description
SCARA5: Ferritin receptor that mediates non-transferrin- dependent delivery of iron. Mediates cellular uptake of ferritin- bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type- specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1). Belongs to the SCARA5 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 8p21.1
Cellular Component: cell surface; integral to plasma membrane
Molecular Function: scavenger receptor activity
Biological Process: receptor-mediated endocytosis; cellular iron ion homeostasis; regulation of gene expression; regulation of blood coagulation; endocytosis
Research Articles on SCARA5
Similar Products
Product Notes
The Human SCARA5 scara5 (Catalog #AAA9714412) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714412 ELISA Kit recognizes Human SCARA5. It is sometimes possible for the material contained within the vial of "Scavenger Receptor Class A Member 5 (SCARA5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
