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Typical Testing Data/Standard Curve (for reference only)

Human Trichohyalin (TCHH) ELISA Kit | TCHH elisa kit

Human Trichohyalin (TCHH) ELISA Kit

Gene Names
TCHH; THH; THL; TRHY
Reactivity
Human
Synonyms
Trichohyalin (TCHH); Human Trichohyalin (TCHH) ELISA Kit; THH; THL; TRHY; TCHH elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Trichohyalin (TCHH).
No significant cross-reactivity or interference between Trichohyalin (TCHH) and analogues was observed.
Sequence Length
1,943
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Trichohyalin (TCHH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Trichohyalin (TCHH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TCHH elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trichohyalin (TCHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trichohyalin (TCHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trichohyalin (TCHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Trichohyalin (TCHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TCHH elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
253,925 Da
NCBI Official Full Name
trichohyalin
NCBI Official Synonym Full Names
trichohyalin
NCBI Official Symbol
TCHH
NCBI Official Synonym Symbols
THH; THL; TRHY
NCBI Protein Information
trichohyalin
UniProt Protein Name
Trichohyalin
Protein Family
UniProt Gene Name
TCHH
UniProt Synonym Gene Names
THH; THL; TRHY
UniProt Entry Name
TRHY_HUMAN

NCBI Description

Trichohyalin confers mechanical strength to the hair follicle inner root sheath and to other toughened epithelial tissues, such as the hard palate and filiform ridges of the tongue, by forming multiple complex crosslinks with itself and with other structural proteins (Steinert et al., 2003 [PubMed 12853460]).[supplied by OMIM, Dec 2009]

Uniprot Description

THH: Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation. Belongs to the S100-fused protein family.

Protein type: Adaptor/scaffold; Calcium-binding

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoskeleton

Molecular Function: calcium ion binding

Biological Process: keratinization

Research Articles on TCHH

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Product Notes

The Human TCHH tchh (Catalog #AAA2023135) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023135 ELISA Kit recognizes Human TCHH. It is sometimes possible for the material contained within the vial of "Trichohyalin (TCHH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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