Human Trichohyalin (TCHH) ELISA Kit | TCHH elisa kit

Human Trichohyalin (TCHH) ELISA Kit

Cat. Num. AAA455038

• Gene Names: TCHH; THH; THL; TRHY; UHS3
• Reactivity: Human
• Synonyms: Trichohyalin (TCHH); Human Trichohyalin (TCHH) ELISA Kit; TCHH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TCHH. No significant cross-reactivity or interference between TCHH and analogues was observed.
• Sequence Length: 1943

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TCHH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TCHH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TCHH elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TCHH in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TCHH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TCHH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TCHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TCHH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148746195
• NCBI GeneID: 7062
• NCBI Accession #: NP_009044.2
• NCBI GenBank Nucleotide #: NM_007113.3
• UniProt Accession #: Q07283; Q5VUI3
• Molecular Weight: 253,925 Da
• NCBI Official Full Name: trichohyalin
• NCBI Official Synonym Full Names: trichohyalin
• NCBI Official Symbol: TCHH
• NCBI Official Synonym Symbols: THH; THL; TRHY; UHS3
• NCBI Protein Information: trichohyalin
• UniProt Protein Name: Trichohyalin
• Protein Family: Trichohyalin
• UniProt Gene Name: TCHH
• UniProt Synonym Gene Names: THH; THL; TRHY

NCBI Description

Trichohyalin confers mechanical strength to the hair follicle inner root sheath and to other toughened epithelial tissues, such as the hard palate and filiform ridges of the tongue, by forming multiple complex crosslinks with itself and with other structural proteins (Steinert et al., 2003 [PubMed 12853460]).[supplied by OMIM, Dec 2009]

Uniprot Description

Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.