Human Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) ELISA Kit | TRPC6 elisa kit

Human Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) ELISA Kit

Cat. Num. AAA2705930

• Gene Names: TRPM6; HSH; HMGX; HOMG; CHAK2; HOMG1
• Reactivity: Human
• Synonyms: Transient Receptor Potential Cation Channel Subfamily C; Member 6 (TRPC6); Human Transient Receptor Potential Cation Channel Subfamily C; Member 6 (TRPC6) ELISA Kit; FSGS2; TRP6; Transient receptor protein 6; TRPC6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6).; No significant cross-reactivity or interference between Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) and analogues was observed.
• Sequence Length: 2017

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRPC6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 293597574
• NCBI GeneID: 140803
• NCBI Accession #: NP_001170782.1
• NCBI GenBank Nucleotide #: NM_001177311.1
• UniProt Accession #: Q9BX84; Q6VPR8; Q6VPR9; Q6VPS0; Q6VPS1; Q6VPS2
• Molecular Weight: 65,083 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 6 isoform c
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 6
• NCBI Official Symbol: TRPM6
• NCBI Official Synonym Symbols: HSH; HMGX; HOMG; CHAK2; HOMG1
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 6
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 6
• Protein Family: Short transient receptor potential channel
• UniProt Gene Name: TRPM6
• UniProt Synonym Gene Names: CHAK2

NCBI Description

This gene is predominantly expressed in the kidney and colon, and encodes a protein containing an ion channel domain and a protein kinase domain. It is crucial for magnesium homeostasis, and plays an essential role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Mutations in this gene are associated with hypomagnesemia with secondary hypocalcemia. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

ChaK2: is an unusual bifunctional protein containing a transient receptor potential (TRP) ion channel fused to a protein kinase of the Atypical alpha group. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. ChaK2 mutations cause hereditary hypomagnesemia and secondary hypocalcemia (HSH). The TRP voltage-independent calcium-permeable cation channels are divided into six subfa milies, two of which (TRPC and TRPM) have members that are widely expressed and activated by oxidative stress. Related to ChaK1, another Atypical alpha kinase fused to a TRP ion channel protein. Forms homodimers or heterodimers with ChaK1. Six alternatively spliced isoforms have been described, three of which lack the ion channel region.

Protein type: ATYPICAL group; Alpha family; ChaK subfamily; Channel, cation; EC 2.7.11.1; Kinase, protein; Membrane protein, integral; Membrane protein, multi-pass; Protein kinase, atypical

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: apical plasma membrane; brush border membrane; integral component of membrane; plasma membrane

Molecular Function: ATP binding; calcium channel activity; metal ion binding; protein binding; protein serine/threonine kinase activity

Biological Process: protein amino acid phosphorylation; protein tetramerization; response to toxin

Disease: Hypomagnesemia 1, Intestinal