Choline Kinase B Active Protein | CHKB active protein

Choline Kinase B, Human Recombinant

Cat. Num. AAA849847

• Gene Names: CHKB; CK; EK; CKB; EKB; CHKL; CHETK; CKEKB; MDCMC
• Purity: >90% by SDS-PAGE
• Synonyms: Choline Kinase B; Human Recombinant; CHKB; choline kinase beta; ATP:choline phosphotransferase B; CHKB active protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris, pH8.0, 150mM NaCl, 2mM DTT, 2mM EDTA and 20% glycerol

• Gene Source: Human
• Biological Activity: Specific activity >=0.05 unit/mg; Measured by the amount of enzyme that catalyzes the phosphorylation of 1 umole of choline to phosphocholine per minute at pH 8 and 37 degree C.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Testing Data

Testing Data

Related Product Information for CHKB active protein

Choline Kinase (E.C. 2.7.1.32) is an enzyme which catalyzes the first reaction in the choline pathway for the synthesis of the membrane phospholipid phosphatidylcholine. It belongs to the family of transferase and uses Mg2+ as a cofactor to transfer a phosphate group from ATP to choline to form phosphocholine. Choline Kinase plays a critical role in cell growth. In humans, choline kinase A and B are present and they share >60% homology. Both of them are implicated in different human diseases as choline kinase A is suggested to associate with breast, lung, colorectal, prostate and bladder cancers due to its high expression in different cancer tissues whereas choline kinase B is suggested to be involved in muscular dystrophy. active human choline kinase B is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery. Plays a key role in phospholipid biosynthesis

Product Categories/Family for CHKB active protein

Neuroscience Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6978649
• NCBI GeneID: 1120
• NCBI Accession #: NP_005189.2
• NCBI GenBank Nucleotide #: NM_005198.4
• UniProt Accession #: Q9Y259; Q13388; A0PJM6
• Molecular Weight: 45,271 Da
• NCBI Official Full Name: choline/ethanolamine kinase
• NCBI Official Synonym Full Names: choline kinase beta
• NCBI Official Symbol: CHKB
• NCBI Official Synonym Symbols: CK; EK; CKB; EKB; CHKL; CHETK; CKEKB; MDCMC
• NCBI Protein Information: choline/ethanolamine kinase; ethanolamine kinase beta; choline kinase-like protein
• UniProt Protein Name: Choline/ethanolamine kinase
• Protein Family: Choline kinase
• UniProt Gene Name: CHKB
• UniProt Synonym Gene Names: CHETK; CHKL; CK; CKB; EK; EKB; CKEKB
• UniProt Entry Name: CHKB_HUMAN

NCBI Description

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]

Uniprot Description

CHKB: Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; EC 2.7.1.82; Lipid Metabolism - glycerophospholipid; EC 2.7.1.32

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: choline kinase activity; ethanolamine kinase activity; ATP binding

Biological Process: CDP-choline pathway; phospholipid metabolic process; phosphatidylethanolamine biosynthetic process; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; phosphorylation

Disease: Muscular Dystrophy, Congenital, Megaconial Type