Human Traf2 binding protein (T2BP) ELISA Kit | T2BP elisa kit

Human Traf2 binding protein (T2BP) ELISA Kit

Cat. Num. AAA287437

• Gene Names: TIFA; T2BP; T6BP; TIFAA
• Reactivity: Human
• Synonyms: Traf2 binding protein (T2BP); Human Traf2 binding protein (T2BP) ELISA Kit; MGC20791; T2BP; T6BP; TIFAA; TRAF-interacting protein with a forkhead-associated domain; TRAF2 binding protein; TRAF6 binding protein; T2BP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TIFA. No significant cross-reactivity or interference between Human TIFA and analogues was observed.
• Sequence Length: 184

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for T2BP elisa kit

This assay employs a two-site sandwich ELISA to quantitate TIFA in samples. An antibody specific for TIFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIFA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIFA bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 33563370
• NCBI GeneID: 92610
• NCBI Accession #: NP_443096.1
• NCBI GenBank Nucleotide #: NM_052864.2
• UniProt Accession #: Q96CG3
• Molecular Weight: 21,445 Da
• NCBI Official Full Name: TRAF-interacting protein with FHA domain-containing protein A
• NCBI Official Synonym Full Names: TRAF interacting protein with forkhead associated domain
• NCBI Official Symbol: TIFA
• NCBI Official Synonym Symbols: T2BP; T6BP; TIFAA
• NCBI Protein Information: TRAF-interacting protein with FHA domain-containing protein A
• UniProt Protein Name: TRAF-interacting protein with FHA domain-containing protein A
• UniProt Gene Name: TIFA
• UniProt Synonym Gene Names: T2BP

Uniprot Description

Adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. Induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism.