Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) Standard Curve.)
Mouse TRAF-Interacting Protein With FHA Domain-Containing Protein A (TIFA) ELISA Kit | TIFA elisa kit
Mouse TRAF-Interacting Protein With FHA Domain-Containing Protein A (TIFA) ELISA Kit
Gene Names
Tifa; T2bp
Reactivity
Mouse
Synonyms
TRAF-Interacting Protein With FHA Domain-Containing Protein A (TIFA); Mouse TRAF-Interacting Protein With FHA Domain-Containing Protein A (TIFA) ELISA Kit; TIFA; MGC20791; T2BP; T6BP; TIFAA; TRAF-interacting protein with a forkhead-associated domain; TRAF2 binding protein; TRAF6 binding protein; TIFA elisa kit
Reactivity
Mouse
Specificity
Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIFA. No significant cross-reactivity or interference between Mouse TIFA and analogues was observed.
Sequence Length
184
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) Standard Curve.)
Related Product Information for TIFA elisa kit
Description: This Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) ELISA Kit employs a two-site sandwich ELISA to quantitate TIFA in samples. An antibody specific for TIFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIFA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIFA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: IL-22, a class of potent mediators of cellular inflammatory responses. It shares us of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28, IL-29. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1.
Background: IL-22, a class of potent mediators of cellular inflammatory responses. It shares us of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28, IL-29. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
TRAF-interacting protein with FHA domain-containing protein A
NCBI Official Synonym Full Names
TRAF-interacting protein with forkhead-associated domain
NCBI Official Symbol
Tifa
NCBI Official Synonym Symbols
T2bp
NCBI Protein Information
TRAF-interacting protein with FHA domain-containing protein A
UniProt Protein Name
TRAF-interacting protein with FHA domain-containing protein A
Protein Family
UniProt Gene Name
Tifa
UniProt Synonym Gene Names
T2bp
UniProt Entry Name
TIFA_MOUSE
Research Articles on TIFA
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Product Notes
The Mouse TIFA tifa (Catalog #AAA9718025) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718025 ELISA Kit recognizes Mouse TIFA. It is sometimes possible for the material contained within the vial of "TRAF-Interacting Protein With FHA Domain-Containing Protein A (TIFA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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