Human Tolloid Like Protein 1 (TLL1) ELISA Kit | TLL1 elisa kit

Human Tolloid Like Protein 1 (TLL1) ELISA Kit

Cat. Num. AAA455627

• Gene Names: TLL1; TLL; ASD6
• Reactivity: Human
• Synonyms: Tolloid Like Protein 1 (TLL1); Human Tolloid Like Protein 1 (TLL1) ELISA Kit; TLL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TLL1. No significant cross-reactivity or interference between TLL1 and analogues was observed.
• Sequence Length: 392

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.216ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TLL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TLL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLL1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLL1 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TLL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TLL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TLL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 325652110
• NCBI GeneID: 7092
• NCBI Accession #: NP_001191689.1
• NCBI GenBank Nucleotide #: NM_001204760.1
• UniProt Accession #: O43897; Q96AN3; Q9NQS4; B2RMU2
• Molecular Weight: 44,186 Da
• NCBI Official Full Name: tolloid-like protein 1 isoform 2
• NCBI Official Synonym Full Names: tolloid like 1
• NCBI Official Symbol: TLL1
• NCBI Official Synonym Symbols: TLL; ASD6
• NCBI Protein Information: tolloid-like protein 1
• UniProt Protein Name: Tolloid-like protein 1
• Protein Family: Tolloid-like protein
• UniProt Gene Name: TLL1
• UniProt Synonym Gene Names: TLL

NCBI Description

This gene encodes an astacin-like, zinc-dependent, metalloprotease that belongs to the peptidase M12A family. This protease processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Studies in mice suggest that this gene plays multiple roles in the development of mammalian heart, and is essential for the formation of the interventricular septum. Allelic variants of this gene are associated with atrial septal defect type 6. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

Protease which processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Required for the embryonic development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis.