Human Tolloid Like Protein 1 (TLL1) ELISA Kit | TLL1 elisa kit

Human Tolloid Like Protein 1 (TLL1) ELISA Kit

Cat. Num. AAA2022475

• Gene Names: TLL1; TLL; ASD6
• Reactivity: Human
• Synonyms: Tolloid Like Protein 1 (TLL1); Human Tolloid Like Protein 1 (TLL1) ELISA Kit; TLL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tolloid Like Protein 1 (TLL1).; No significant cross-reactivity or interference between Tolloid Like Protein 1 (TLL1) and analogues was observed.
• Sequence Length: 392

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.216ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tolloid Like Protein 1 (TLL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tolloid Like Protein 1 (TLL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tolloid Like Protein 1 (TLL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tolloid Like Protein 1 (TLL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tolloid Like Protein 1 (TLL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tolloid Like Protein 1 (TLL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TLL1 elisa kit

Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 22547221
• NCBI GeneID: 7092
• NCBI Accession #: NP_036596.3
• NCBI GenBank Nucleotide #: NM_012464.4
• UniProt Accession #: O43897; Q96AN3; Q9NQS4; B2RMU2
• Molecular Weight: 44,186 Da
• NCBI Official Full Name: tolloid-like protein 1 isoform 1
• NCBI Official Synonym Full Names: tolloid-like 1
• NCBI Official Symbol: TLL1
• NCBI Official Synonym Symbols: TLL; ASD6
• NCBI Protein Information: tolloid-like protein 1
• UniProt Protein Name: Tolloid-like protein 1
• Protein Family: Tolloid-like protein
• UniProt Gene Name: TLL1
• UniProt Synonym Gene Names: TLL
• UniProt Entry Name: TLL1_HUMAN

NCBI Description

This gene encodes an astacin-like, zinc-dependent, metalloprotease that belongs to the peptidase M12A family. This protease processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Studies in mice suggest that this gene plays multiple roles in the development of mammalian heart, and is essential for the formation of the interventricular septum. Allelic variants of this gene are associated with atrial septal defect type 6. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

TLL1: Protease which processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Required for the embryonic development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis. Defects in TLL1 are the cause of atrial septal defect type 6 (ASD6). A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Belongs to the peptidase M12A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 4q32-q33

Cellular Component: extracellular region

Molecular Function: collagen binding; zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell differentiation; proteolysis; skeletal development

Disease: Atrial Septal Defect 6