Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anti-tissue transglutaminase IgA (IgA-tTG) Standard Curve.)
Human Tissue transglutaminase IgA (IgA-tTG) ELISA Kit | IgA-tTG elisa kit
Human Anti-Tissue transglutaminase IgA (IgA-tTG) ELISA Kit
Reactivity
Human
Synonyms
Tissue transglutaminase IgA (IgA-tTG); Human Anti-Tissue transglutaminase IgA (IgA-tTG) ELISA Kit; IgA-tTG elisa kit
Reactivity
Human
Specificity
Human Anti-tissue transglutaminase IgA (IgA-tTG) ELISA Kit has high sensitivity and excellent specificity for detection of Human IgA-tTG. No significant cross-reactivity or interference between Human IgA-tTG and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anti-tissue transglutaminase IgA (IgA-tTG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anti-tissue transglutaminase IgA (IgA-tTG) Standard Curve.)
Related Product Information for IgA-tTG elisa kit
Description: This Human Anti-tissue transglutaminase IgA (IgA-tTG) ELISA Kit employs a two-site sandwich ELISA to quantitate IgA-tTG in samples. An antibody specific for IgA-tTG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA-tTG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IgA-tTG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgA-tTG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tissue transglutaminase (abbreviated as tTG or TG2) is a 78-kDa, calcium-dependent enzyme (EC 2.3.2.13) of the protein-glutamine gamma-glutamyltransferases family (or simply transglutaminase family). Like other transglutaminases, it crosslinks proteins between an epsilon-amino group of a lysine residue and a gamma-carboxamide group of glutamine residue, creating an inter- or intramolecular bond that is highly resistant to proteolysis (protein degradation). Aside from its crosslinking function, tTG catalyzes other types of reactions including deamidation, GTP-binding/hydrolyzing, and isopeptidase activities. Unlike other members of the transglutaminase family, tTG can be found both in the intracellular and the extracellular spaces of various types of tissues and is found in many different organs including the heart, the liver, and the small intestine. Intracellular tTG is abundant in the cytosol but smaller amounts can also be found in the nucleus and the mitochondria. Intracellular tTG is thought to play an important role in apoptosis. In the extracellular space, tTG binds to proteins of the extracellular matrix (ECM), binding particularly tightly to fibronectin. Extracellular tTG has been linked to cell adhesion, ECM stabilization, wound healing, receptor signaling, cellular proliferation, and cellular motility. tTG is particularly notable for being the autoantigen in celiac disease, a lifelong illness in which the consumption of dietary gluten causes a pathological immune response resulting in the inflammation of the small intestine and subsequent villous atrophy.
Background: Tissue transglutaminase (abbreviated as tTG or TG2) is a 78-kDa, calcium-dependent enzyme (EC 2.3.2.13) of the protein-glutamine gamma-glutamyltransferases family (or simply transglutaminase family). Like other transglutaminases, it crosslinks proteins between an epsilon-amino group of a lysine residue and a gamma-carboxamide group of glutamine residue, creating an inter- or intramolecular bond that is highly resistant to proteolysis (protein degradation). Aside from its crosslinking function, tTG catalyzes other types of reactions including deamidation, GTP-binding/hydrolyzing, and isopeptidase activities. Unlike other members of the transglutaminase family, tTG can be found both in the intracellular and the extracellular spaces of various types of tissues and is found in many different organs including the heart, the liver, and the small intestine. Intracellular tTG is abundant in the cytosol but smaller amounts can also be found in the nucleus and the mitochondria. Intracellular tTG is thought to play an important role in apoptosis. In the extracellular space, tTG binds to proteins of the extracellular matrix (ECM), binding particularly tightly to fibronectin. Extracellular tTG has been linked to cell adhesion, ECM stabilization, wound healing, receptor signaling, cellular proliferation, and cellular motility. tTG is particularly notable for being the autoantigen in celiac disease, a lifelong illness in which the consumption of dietary gluten causes a pathological immune response resulting in the inflammation of the small intestine and subsequent villous atrophy.
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Product Notes
The Human IgA-tTG (Catalog #AAA9716208) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716208 ELISA Kit recognizes Human IgA-tTG. It is sometimes possible for the material contained within the vial of "Tissue transglutaminase IgA (IgA-tTG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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