Typical Testing Data/Standard Curve (for reference only)
Human Thymosin Beta 4 (Tb4) ELISA Kit | Tb4 elisa kit
Human Thymosin Beta 4 (Tb4) ELISA Kit
Gene Names
TMSB4X; FX; TB4X; PTMB4; TMSB4
Reactivity
Human
Synonyms
Thymosin Beta 4 (Tb4); Human Thymosin Beta 4 (Tb4) ELISA Kit; TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin; Beta 4; X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide; Tb4 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Tb4. No significant cross-reactivity or interference between Tb4 and analogues was observed.
Sequence Length
44
Samples
Human serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
2.47-200ng/mL
Sensitivity
<0.93ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tb4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tb4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Tb4 elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Tb4 in human serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tb4 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tb4 and unlabeled Tb4 (Standards or samples) with the pre-coated antibody specific to Tb4. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tb4 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tb4 in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tb4 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tb4 and unlabeled Tb4 (Standards or samples) with the pre-coated antibody specific to Tb4. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tb4 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tb4 in the sample.
Product Categories/Family for Tb4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
5,053 Da
NCBI Official Full Name
thymosin beta-4
NCBI Official Synonym Full Names
thymosin beta 4, X-linked
NCBI Official Symbol
TMSB4X
NCBI Official Synonym Symbols
FX; TB4X; PTMB4; TMSB4
NCBI Protein Information
thymosin beta-4
UniProt Protein Name
Thymosin beta-4
UniProt Gene Name
TMSB4X
UniProt Synonym Gene Names
TB4X; THYB4; TMSB4; T beta-4
UniProt Entry Name
TYB4_HUMAN
NCBI Description
This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]
Uniprot Description
TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: Xq21.3-q22
Cellular Component: cytoskeleton; extracellular region
Molecular Function: actin monomer binding; protein binding
Biological Process: platelet activation; platelet degranulation; sequestering of actin monomers; actin filament organization; blood coagulation
Research Articles on Tb4
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Product Notes
The Human Tb4 tmsb4x (Catalog #AAA2024602) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024602 ELISA Kit recognizes Human Tb4. It is sometimes possible for the material contained within the vial of "Thymosin Beta 4 (Tb4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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