Rat Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) RTU ELISA Kit | MYH7 rtu elisa kit

Rat Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) ELISA Kit

Cat. Num. AAA4501250

• Gene Names: Myh7; Bmyo; Myhcb; myHC-beta; myHC-slow
• Reactivity: Rat
• Synonyms: Myosin Heavy Chain 7; Cardiac Muscle; Beta (MYH7); Rat Myosin Heavy Chain 7; Beta (MYH7) ELISA Kit; CMD1S; CMH1; MPD1; MYHCB; Myopathy; Distal 1; Myosin heavy chain slow isoform; Myosin heavy chain; cardiac muscle beta isoform; MYH7 rtu elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of MYH7. No significant cross-reactivity or interference between MYH7 and analogues was observed.
• Sequence Length: 1935

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 125-8000pg/mL
• Sensitivity: < 43pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MYH7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MYH7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH7 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MYH7 in rat serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYH7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MYH7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYH7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MYH7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393807
• NCBI GeneID: 29557
• NCBI Accession #: NP_058936.1
• NCBI GenBank Nucleotide #: NM_017240.2
• UniProt Accession #: P02564
• Molecular Weight: 223,083 Da
• NCBI Official Full Name: myosin-7
• NCBI Official Synonym Full Names: myosin heavy chain 7
• NCBI Official Symbol: Myh7
• NCBI Official Synonym Symbols: Bmyo; Myhcb; myHC-beta; myHC-slow
• NCBI Protein Information: myosin-7
• UniProt Protein Name: Myosin-7
• Protein Family: Myosin
• UniProt Gene Name: Myh7
• UniProt Synonym Gene Names: MyHC-slow; MyHC-beta

NCBI Description

heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

Uniprot Description

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.