Typical Testing Data/Standard Curve (for reference only)
Human Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) ELISA Kit | THSD7A elisa kit
Human Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) ELISA Kit
Reactivity
Human
Synonyms
Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A); Human Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A) ELISA Kit; THSD7A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-THSD7A. No significant cross-reactivity or interference between Anti-THSD7A and analogues was observed.
Sequence Length
1657
Samples
Serum, plasma and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
< 6.7pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-THSD7A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-THSD7A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for THSD7A elisa kit
Intended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-THSD7A in human serum, plasma and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with THSD7A. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-THSD7A will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-THSD7A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with THSD7A. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-THSD7A will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-THSD7A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for THSD7A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
185,363 Da
NCBI Official Full Name
thrombospondin type-1 domain-containing protein 7A
NCBI Official Synonym Full Names
thrombospondin type 1 domain containing 7A
NCBI Official Symbol
THSD7A
NCBI Protein Information
thrombospondin type-1 domain-containing protein 7A
UniProt Protein Name
Thrombospondin type-1 domain-containing protein 7A
Protein Family
UniProt Gene Name
THSD7A
UniProt Synonym Gene Names
KIAA0960
NCBI Description
The protein encoded by this gene is found almost exclusively in endothelial cells from placenta and umbilical cord. The encoded protein appears to interact with alpha(V)beta(3) integrin and paxillin to inhibit endothelial cell migration and tube formation. This protein may be involved in cytoskeletal organization. Variations in this gene may be associated with low bone mineral density in osteoporosis. [provided by RefSeq, Aug 2010]
Uniprot Description
THSD7A: The soluble form promotes endothelial cell migration and filopodia formationduring angiogenesis via a FAK-dependent mechanism.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 7p21.3
Cellular Component: integral component of membrane; plasma membrane
Biological Process: angiogenesis; cell differentiation
Research Articles on THSD7A
Similar Products
Product Notes
The Human THSD7A thsd7a (Catalog #AAA2097746) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2097746 ELISA Kit recognizes Human THSD7A. It is sometimes possible for the material contained within the vial of "Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody (Anti-THSD7A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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