Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thioredoxin reductase 2, mitochondrial (TXNRD2) Standard Curve.)
Human Thioredoxin Reductase 2, Mitochondrial (TXNRD2) ELISA Kit | TXNRD2 elisa kit
Human Thioredoxin Reductase 2, Mitochondrial (TXNRD2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thioredoxin reductase 2, mitochondrial (TXNRD2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thioredoxin reductase 2, mitochondrial (TXNRD2) Standard Curve.)
Background: Thioredoxin reductase (TR) is a dimeric NADPH-dependent FAD containing enzyme that catalyzes the reduction of the active site disulfide of thioredoxin and other substrates. TR is a member of a family of pyridine nucleotide-disulfide oxidoreductases and is a key enzyme in the regulation of the intracellular redox environment. Three thioredoxin reductase genes have been found that encode selenocysteine containing proteins. TrxR2 partially overlaps the COMT gene on chromosome 22.Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene. [provided by RefSeq, Jun 2017]
Uniprot Description
TXNRD2: Maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox- regulated cell signaling. Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleotide Metabolism - pyrimidine; EC 1.8.1.9; Nuclear receptor co-regulator; Oxidoreductase
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: mitochondrion; mitochondrial matrix
Molecular Function: protein binding; thioredoxin-disulfide reductase activity; mercury (II) reductase activity; FAD binding; mercury ion binding; NADP binding
Biological Process: response to reactive oxygen species; response to oxygen radical; cell redox homeostasis; detoxification of mercury ion
Research Articles on TXNRD2
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Product Notes
The Human TXNRD2 txnrd2 (Catalog #AAA9713779) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713779 ELISA Kit recognizes Human TXNRD2. It is sometimes possible for the material contained within the vial of "Thioredoxin Reductase 2, Mitochondrial (TXNRD2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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