Human Thiopurine S-Methyltransferase (TPMT) ELISA Kit | TPMT elisa kit
Human Thiopurine S-Methyltransferase (TPMT) ELISA Kit
Reactivity
Human
Synonyms
Thiopurine S-Methyltransferase (TPMT); Human Thiopurine S-Methyltransferase (TPMT) ELISA Kit; TPMT elisa kit
Reactivity
Human
Sequence Length
259
Assay Type
Sandwich ELISA
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Range
15 pg/mL-240 pg/mL
Detection Method
Colorimetric
Limit of Detection
1.0 pg/mL
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for TPMT elisa kit
Human Thiopurine S-methyltransferase (TPMT) ELISA Kit employs a two-site sandwich ELISA to quantitate TPMT in samples. An antibody specific for TPMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPMT present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human TPMT detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TPMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
thiopurine S-methyltransferase
NCBI Official Synonym Full Names
thiopurine S-methyltransferase
NCBI Official Symbol
Tpmt
NCBI Protein Information
thiopurine S-methyltransferase
NCBI Description
structural protein of tight junctions; expression in brain is reduced in diabetic rats [RGD, Dec 2006]
Research Articles on TPMT
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Product Notes
The Human TPMT (Catalog #AAA9713816) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713816 ELISA Kit recognizes Human TPMT. It is sometimes possible for the material contained within the vial of "Thiopurine S-Methyltransferase (TPMT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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