Rabbit anti-Human, Mouse Raf1 Polyclonal Antibody | anti-Raf1 antibody

Raf1, NT (Raf1, Craf, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, Raf-1) (AP)

Cat. Num. AAA6340097

• Gene Names: Raf1; Craf1; Raf-1; c-Raf; v-Raf; AA990557; BB129353; 6430402F14Rik; D830050J10Rik
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Raf1; Polyclonal Antibody; NT (Raf1; Craf; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; Raf-1) (AP); Raf-1; anti-Raf1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Raf1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Raf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-113 amino acids from the N-terminal region of Mouse Raf1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Raf1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Raf1 antibody detected the Raf1 protein (arrow).)

Western Blot (WB)

(Mouse Raf1 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Raf1 antibody detected the Raf1 protein (arrow).)

Flow Cytometry (FC/FACS)

(Mouse Raf1 Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Mouse Raf1 Antibody (N-term) (AP14280a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Raf1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Raf1 antibody

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3 (By similarity).

Product Categories/Family for anti-Raf1 antibody

Antibodies; Protein Kinases

References

Niault, T.S., et al. Carcinogenesis 31(7):1165-1174(2010). Batarseh, A., et al. Biochemistry 49(23):4766-4778(2010). Tarutani, M., et al. J. Dermatol. Sci. 58(1):28-35(2010). Heidorn, S.J., et al. Cell 140(2):209-221(2010). Niault, T., et al. J. Cell Biol. 187(3):335-342(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 18497290
• NCBI GeneID: 110157
• NCBI Accession #: NP_084056.1
• NCBI GenBank Nucleotide #: NM_029780.3
• UniProt Accession #: Q99N57; Q3UR68; Q58E75; Q91WH1; Q99N58; Q9QUU8
• Molecular Weight: 72,917 Da
• NCBI Official Full Name: RAF proto-oncogene serine/threonine-protein kinase
• NCBI Official Synonym Full Names: v-raf-leukemia viral oncogene 1
• NCBI Official Symbol: Raf1
• NCBI Official Synonym Symbols: Craf1; Raf-1; c-Raf; v-Raf; AA990557; BB129353; 6430402F14Rik; D830050J10Rik
• NCBI Protein Information: RAF proto-oncogene serine/threonine-protein kinase; cRaf; Craf1 transforming; proto-oncogene c-RAF; sarcoma 3611 oncogene; murine sarcoma 3611 oncogene 1; v-raf-1 leukemia viral oncogene 1
• UniProt Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• Protein Family: Rik1-associated factor
• UniProt Gene Name: Raf1
• UniProt Synonym Gene Names: Craf; cRaf
• UniProt Entry Name: RAF1_MOUSE

Uniprot Description

RAF1: a proto-oncogenic TKL kinase of the RAF family. Functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate and activate MEK1/2, which in turn phosphorylate and activate ERK1/2. Acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MEK1/2) and the extracellular signal-regulated kinases (ERK1/2). RAF1, subsequent to phosphorylation by PAK1, phosphorylates BAD (Bcl2-antagonist of cell death) at S75. Phosphorylates adenylyl cyclases ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2 (cardiac muscle troponin T). Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (ASK1 and MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Oncoprotein; EC 2.7.11.1; TKL group; RAF family

Cellular Component: Golgi apparatus; membrane; mitochondrion; cytoplasm; plasma membrane; pseudopodium; nucleus; cytosol

Molecular Function: transferase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; Ras GTPase binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; metal ion binding; receptor signaling protein activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: activation of MAPKK activity; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; MAPKKK cascade; intermediate filament cytoskeleton organization and biogenesis; signal transduction; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; negative regulation of cell proliferation; response to hypoxia; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; cell differentiation; phosphorylation; negative regulation of protein complex assembly; negative regulation of apoptosis