Human TFPI ELISA Kit | TFPI elisa kit

Human TFPI PicoKine ELISA Kit

Cat. Num. AAA176537

• Gene Names: TFPI; EPI; TFI; LACI; TFPI1
• Reactivity: Human
• Synonyms: TFPI; Human TFPI PicoKine ELISA Kit; Tissue factor pathway inhibitor; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); EPI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; TFPI1_HUMAN; TFPI elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human TFPI
• Sequence Length: 304

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human TFPI PicoKine ELISA Kit standard curve )

Related Product Information for TFPI elisa kit

Principle of the assay: human TFPI ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFPI has been precoated onto 96-well plates. Standards(NSO, D29-K282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFPI amount of sample captured in plate.
Background: TFPI(Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

NCBI and Uniprot Product Information

• NCBI GI #: 125932
• NCBI GeneID: 7035
• UniProt Accession #: P10646; O95103; Q53TS4
• Molecular Weight: 28,653 Da
• NCBI Official Full Name: Tissue factor pathway inhibitor
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• NCBI Official Symbol: TFPI
• NCBI Official Synonym Symbols: EPI; TFI; LACI; TFPI1
• NCBI Protein Information: tissue factor pathway inhibitor; anti-convertin; extrinsic pathway inhibitor
• UniProt Protein Name: Tissue factor pathway inhibitor
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI
• UniProt Synonym Gene Names: LACI; TFPI1; TFPI; EPI; LACI
• UniProt Entry Name: TFPI1_HUMAN

NCBI Description

This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]

Uniprot Description

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Secreted; Secreted, signal peptide; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: extracellular space; cell surface; endoplasmic reticulum; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: blood coagulation, extrinsic pathway; blood coagulation