Human Arginase II (Arg2) ELISA Kit | Arg2 elisa kit

Human Arginase II (Arg2) Wide-range ELISA Kit

Cat. Num. AAA2088278

• Reactivity: Human
• Synonyms: Arginase II (Arg2); Human Arginase II (Arg2) Wide-range ELISA Kit; Kidney-type arginase; Non-hepatic arginase; Arg2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Arginase II (Arg2).; No significant cross-reactivity or interference between Wide-range Arginase II (Arg2) and analogues was observed.
• Sequence Length: 354

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.15ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Arginase II (Arg2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Arginase II (Arg2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Arg2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Arginase II (Arg2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Arginase II (Arg2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Arginase II (Arg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wide-range Arginase II (Arg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Arg2 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 4502215
• NCBI GeneID: 384
• NCBI Accession #: NP_001163.1
• NCBI GenBank Nucleotide #: NM_001172.3
• UniProt Accession #: P78540; Q6FHY8; B2R690
• Molecular Weight: 38,578 Da
• NCBI Official Full Name: arginase-2, mitochondrial
• NCBI Official Synonym Full Names: arginase 2
• NCBI Official Symbol: ARG2
• NCBI Protein Information: arginase-2, mitochondrial
• UniProt Protein Name: Arginase-2, mitochondrial
• Protein Family: Arginase
• UniProt Gene Name: ARG2

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ARG2: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. Belongs to the arginase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.3.1; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: arginase activity

Biological Process: nitric oxide biosynthetic process; urea cycle