Western Blot (WB)
(Western blot analysis of DOLK Antibody (Center) in 293 cell line lysates (35ug/lane). DOLK (arrow) was detected using the purified Pab.)
Rabbit anti-Human DOLK Polyclonal Antibody | anti-Dolk antibody
DOLK, ID (Transmembrane Protein 15, Dolichol Kinase, KIAA1094, TMEM15, UNQ2422/PRO4980)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Western Blot (WB)
(Western blot analysis of DOLK Antibody (Center) in 293 cell line lysates (35ug/lane). DOLK (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DOLK Antibody (Center) in 293 cell line lysates (35ug/lane). DOLK (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with DOLK Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with DOLK Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DOLK Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DOLK Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
DOLK: Involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors. Defects in DOLK are the cause of congenital disorder of glycosylation type 1M (CDG1M); also known as dolichol kinase deficiency. CDGs are a family of severe inherited diseases caused by a defect in glycoprotein biosynthesis. They are characterized by under-glycosylated serum glycoproteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. CDG1M is a very severe disorder with death occurring in early infancy. Belongs to the polyprenol kinase family.
Protein type: Kinase, other; Membrane protein, multi-pass; Endoplasmic reticulum; EC 2.7.1.108; Membrane protein, integral
Cellular Component: membrane; endoplasmic reticulum; integral to membrane; integral to endoplasmic reticulum membrane
Molecular Function: transferase activity; dolichol kinase activity; kinase activity
Biological Process: dolichyl monophosphate biosynthetic process; phosphorylation
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Product Notes
The Dolk dolk (Catalog #AAA628140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DOLK, ID (Transmembrane Protein 15, Dolichol Kinase, KIAA1094, TMEM15, UNQ2422/PRO4980) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DOLK can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the Dolk dolk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DOLK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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