Human T-Cell, Immune Regulator 1 (TCIRG1) ELISA Kit | TCIRG1 elisa kit

Human T-Cell, Immune Regulator 1 (TCIRG1) ELISA Kit

Cat. Num. AAA2707075

• Gene Names: TCIRG1; a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa
• Reactivity: Human
• Synonyms: T-Cell; Immune Regulator 1 (TCIRG1); Human T-Cell; Immune Regulator 1 (TCIRG1) ELISA Kit; Stv1; Vph1; ATP6N1C; ATP6V0A3; Atp6i; OC-116kDa; OC116; OPTB1; TIRC7; a3; V-Type Proton ATPase 116 kDa Subunit A Isoform 3; Osteoclastic proton pump 116 kDa subunit; TCIRG1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of T-Cell, Immune Regulator 1 (TCIRG1).; No significant cross-reactivity or interference between T-Cell, Immune Regulator 1 (TCIRG1) and analogues was observed.
• Sequence Length: 614

• Assay Type: Double-antibody Sandwich
• Samples: Serum and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.0pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell, Immune Regulator 1 (TCIRG1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell, Immune Regulator 1 (TCIRG1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TCIRG1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell, Immune Regulator 1 (TCIRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell, Immune Regulator 1 (TCIRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell, Immune Regulator 1 (TCIRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell, Immune Regulator 1 (TCIRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51980727
• NCBI GeneID: 10312
• UniProt Accession #: Q13488; O75877; Q8WVC5
• Molecular Weight: 68,776 Da
• NCBI Official Full Name: T-cell immune regulator 1 transcript variant 4, partial
• NCBI Official Synonym Full Names: T cell immune regulator 1, ATPase H+ transporting V0 subunit a3
• NCBI Official Symbol: TCIRG1
• NCBI Official Synonym Symbols: a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa
• NCBI Protein Information: V-type proton ATPase 116 kDa subunit a isoform 3; V-type proton ATPase 116 kDa subunit a
• UniProt Protein Name: V-type proton ATPase 116 kDa subunit a isoform 3
• Protein Family: V-type proton ATPase
• UniProt Gene Name: TCIRG1
• UniProt Synonym Gene Names: ATP6N1C; ATP6V0A3; V-ATPase 116 kDa isoform a3; OC-116 kDa; OC116; TIRC7

NCBI Description

This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017]

Uniprot Description

Part of the proton channel of V-ATPases (). Seems to be directly involved in T-cell activation.