Human Syntenin 2 (ST2) ELISA Kit | ST2 elisa kit

Human Syntenin 2 (ST2) ELISA Kit

Cat. Num. AAA452375

• Gene Names: SDCBP2; ST-2; SITAC; SITAC18
• Reactivity: Human
• Synonyms: Syntenin 2 (ST2); Human Syntenin 2 (ST2) ELISA Kit; SDCBP2 SITAC18 Syndecan Binding Protein 2; ST2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ST2. No significant cross-reactivity or interference between ST2 and analogues was observed.
• Sequence Length: 292

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.071ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ST2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ST2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ST2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ST2 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ST2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ST2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ST2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ST2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 315139014
• NCBI GeneID: 27111
• NCBI Accession #: NP_001186713.1
• NCBI GenBank Nucleotide #: NM_001199784.1
• UniProt Accession #: Q9H190; O95892; Q5W0X1; Q9BZ42; Q9H567; Q9NRY8
• Molecular Weight: 22,664 Da
• NCBI Official Full Name: syntenin-2 isoform a
• NCBI Official Synonym Full Names: syndecan binding protein 2
• NCBI Official Symbol: SDCBP2
• NCBI Official Synonym Symbols: ST-2; SITAC; SITAC18
• NCBI Protein Information: syntenin-2
• UniProt Protein Name: Syntenin-2
• Protein Family: High affinity sulfate transporter
• UniProt Gene Name: SDCBP2
• UniProt Synonym Gene Names: SITAC18

NCBI Description

The protein encoded by this gene contains two class II PDZ domains. PDZ domains facilitate protein-protein interactions by binding to the cytoplasmic C-terminus of transmembrane proteins, and PDZ-containing proteins mediate cell signaling and the organization of protein complexes. The encoded protein binds to phosphatidylinositol 4, 5-bisphosphate (PIP2) and plays a role in nuclear PIP2 organization and cell division. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Read-through transcription also exists between this gene and the upstream FKBP1A (FK506 binding protein 1A, 12kDa) gene, as represented in GeneID:100528031. [provided by RefSeq, Sep 2011]