Human Survival motor neuron protein, SMN1 ELISA Kit | SMN1 elisa kit

Human Survival motor neuron protein, SMN1 ELISA Kit

Cat. Num. AAA2602673

• Gene Names: SMN1; SMN
• Reactivity: Human
• Synonyms: Survival motor neuron protein; SMN1; Human Survival motor neuron protein; SMN1 ELISA Kit; SMN1 elisa kit

• Reactivity: Human

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SMN1 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human SMN1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 28849927
• NCBI GeneID: 281492
• NCBI Accession #: NP_783632.1
• NCBI GenBank Nucleotide #: NM_175701.1
• UniProt Accession #: O18870; O46481; O62700; Q9TSJ9
• Molecular Weight: 31,327 Da
• NCBI Official Full Name: survival motor neuron protein
• NCBI Official Synonym Full Names: survival of motor neuron 1, telomeric<
• NCBI Official Symbol: SMN1
• NCBI Official Synonym Symbols: SMN
• NCBI Protein Information: survival motor neuron protein
• UniProt Protein Name: Survival motor neuron protein
• Protein Family: Survival motor neuron protein
• UniProt Gene Name: SMN1
• UniProt Synonym Gene Names: SMN
• UniProt Entry Name: SMN_BOVIN

Uniprot Description

Function: The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs)