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Typical Testing Data/Standard Curve (for reference only)

Human Sucrase Isomaltase (SI) ELISA Kit | SI elisa kit

Human Sucrase Isomaltase (SI) ELISA Kit

Reactivity
Human
Synonyms
Sucrase Isomaltase (SI); Human Sucrase Isomaltase (SI) ELISA Kit; Oligosaccharide Alpha-1; 6-Glucosidase; SI elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SI. No significant cross-reactivity or interference between SI and analogues was observed.
Sequence Length
1827
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.107ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SI were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SI were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SI elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SI in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
209,453 Da
NCBI Official Full Name
sucrase-isomaltase, intestinal
NCBI Official Synonym Full Names
sucrase-isomaltase
NCBI Official Symbol
SI
NCBI Protein Information
sucrase-isomaltase, intestinal
UniProt Protein Name
Sucrase-isomaltase, intestinal
Protein Family
UniProt Gene Name
SI

NCBI Description

This gene encodes a sucrase-isomaltase enzyme that is expressed in the intestinal brush border. The encoded protein is synthesized as a precursor protein that is cleaved by pancreatic proteases into two enzymatic subunits sucrase and isomaltase. These two subunits heterodimerize to form the sucrose-isomaltase complex. This complex is essential for the digestion of dietary carbohydrates including starch, sucrose and isomaltose. Mutations in this gene are the cause of congenital sucrase-isomaltase deficiency.[provided by RefSeq, Apr 2010]

Uniprot Description

Plays an important role in the final stage of carbohydrate digestion. Isomaltase activity is specific for both alpha-1,4- and alpha-1,6-oligosaccharides.

Research Articles on SI

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Product Notes

The Human SI si (Catalog #AAA450455) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA450455 ELISA Kit recognizes Human SI. It is sometimes possible for the material contained within the vial of "Sucrase Isomaltase (SI), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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