Human SPTLC1 (Serine palmitoyltransferase 1) ELISA Kit | SPTLC1 elisa kit

Human SPTLC1 (Serine palmitoyltransferase 1) ELISA Kit

Cat. Num. AAA7607195

• Gene Names: Sptlc1; Lcb1; SPT1; C77762; AW552086; E030036H05
• Reactivity: Human
• Synonyms: SPTLC1 (Serine palmitoyltransferase 1); Human SPTLC1 (Serine palmitoyltransferase 1) ELISA Kit; SPTLC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate. No significant cross-reactivity or interference between Sphingosine 1 Phosphate and analogues was observed.
• Sequence Length: 473

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 3.125-200ng/ml
• Sensitivity: <1.875ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine 1 Phosphate were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine 1 Phosphate were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPTLC1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Sphingosine 1 Phosphate antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Sphingosine 1 Phosphate antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Sphingosine 1 Phosphate amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Sphingosine 1 Phosphate can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 29244577
• NCBI GeneID: 268656
• NCBI Accession #: NP_033295.2
• NCBI GenBank Nucleotide #: NM_009269.2
• UniProt Accession #: O35704; O54813; Q8BH11
• Molecular Weight: 52,535 Da
• NCBI Official Full Name: serine palmitoyltransferase 1
• NCBI Official Synonym Full Names: serine palmitoyltransferase, long chain base subunit 1
• NCBI Official Symbol: Sptlc1
• NCBI Official Synonym Symbols: Lcb1; SPT1; C77762; AW552086; E030036H05
• NCBI Protein Information: serine palmitoyltransferase 1
• UniProt Protein Name: Serine palmitoyltransferase 1
• Protein Family: Serine palmitoyltransferase
• UniProt Gene Name: Sptlc1
• UniProt Synonym Gene Names: Lcb1; LCB 1; SPT 1; SPT1

Uniprot Description

Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs ().