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Western Blot (WB) (Western blot analysis of extracts from HepG2 cells and RAW264.7 cells, using NDUFA4 antibody.)

NDUFA4 Antibody | anti-NDUFA4 antibody

NDUFA4 Antibody

Gene Names
NDUFA4; MLRQ; CI-9k; CI-MLRQ
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
NDUFA4; NDUFA4 Antibody; CI-MLRQ; complex I-MLRQ; EC 1.6.5.3; EC 1.6.99.3; MLRQ; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex; 4 (9kD; MLRQ); 4; 9kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex 4; NADH-ubiquinone oxidoreductase MLRQ; NDUA4; anti-NDUFA4 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
NDUFA4 antibody detects endogenous levels of total NDUFA4 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
81
Applicable Applications for anti-NDUFA4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human NDUFA4.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells and RAW264.7 cells, using NDUFA4 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells and RAW264.7 cells, using NDUFA4 antibody.)
References
Kim J.W., Biochem. Mol. Biol. Int. 43:669-675(1997). Kanagarajah D., Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases. Ebert L., Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,370 Da
NCBI Official Full Name
cytochrome c oxidase subunit NDUFA4
NCBI Official Synonym Full Names
NDUFA4, mitochondrial complex associated
NCBI Official Symbol
NDUFA4
NCBI Official Synonym Symbols
MLRQ; CI-9k; CI-MLRQ
NCBI Protein Information
cytochrome c oxidase subunit NDUFA4
UniProt Protein Name
Cytochrome c oxidase subunit NDUFA4
Protein Family
UniProt Gene Name
NDUFA4
UniProt Synonym Gene Names
CI-MLRQ

NCBI Description

The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance.

Research Articles on NDUFA4

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Product Notes

The NDUFA4 ndufa4 (Catalog #AAA855763) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The NDUFA4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the NDUFA4 ndufa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFA4, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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