Human SOCS4 (Suppressor Of Cytokine Signaling 4) ELISA Kit | SOCS4 elisa kit

Human SOCS4 (Suppressor Of Cytokine Signaling 4) ELISA Kit

Cat. Num. AAA7606586

• Gene Names: SOCS4; SOCS7
• Reactivity: Human
• Synonyms: SOCS4 (Suppressor Of Cytokine Signaling 4); Human SOCS4 (Suppressor Of Cytokine Signaling 4) ELISA Kit; Suppressor Of Cytokine Signaling 4; SOCS4 elisa kit

• Reactivity: Human
• Sequence Length: 440

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 3.906--250pg/ml
• Sensitivity: 2.344pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOCS4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SOCS4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SOCS4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SOCS4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SOCS4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20178106
• NCBI GeneID: 122809
• UniProt Accession #: Q8WXH5
• Molecular Weight: 50,623 Da
• NCBI Official Full Name: Suppressor of cytokine signaling 4
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 4
• NCBI Official Symbol: SOCS4
• NCBI Official Synonym Symbols: SOCS7
• NCBI Protein Information: suppressor of cytokine signaling 4
• UniProt Protein Name: Suppressor of cytokine signaling 4
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS4
• UniProt Synonym Gene Names: SOCS7; SOCS-4; SOCS-7

NCBI Description

The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS), also known as STAT-induced STAT inhibitor (SSI), protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SOCS4: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits for instance EGF signaling by mediating the degradation of the EGF receptor/EGFR.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: cytoplasm

Molecular Function: protein binding; protein kinase inhibitor activity

Biological Process: cytokine and chemokine mediated signaling pathway; negative regulation of epidermal growth factor receptor activity; negative regulation of JAK-STAT cascade