Human SOCS1 (Suppressor of Cytokine Signaling 1) ELISA Kit | SOCS1 elisa kit

Human SOCS1 (Suppressor of Cytokine Signaling 1) ELISA Kit

Cat. Num. AAA2540320

• Gene Names: SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
• Reactivity: Human
• Synonyms: SOCS1 (Suppressor of Cytokine Signaling 1); Human SOCS1 (Suppressor of Cytokine Signaling 1) ELISA Kit; SOCS1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human SOCS1. No significant cross-reactivity or interference between Human SOCS1 and analogues was observed.
• Sequence Length: 211

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOCS1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SOCS1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SOCS1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SOCS1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SOCS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SOCS1. You can calculate the concentration of SOCS1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507233
• NCBI GeneID: 8651
• NCBI Accession #: NP_003736.1
• NCBI GenBank Nucleotide #: NM_003745.1
• Molecular Weight: 23,551 Da
• NCBI Official Full Name: suppressor of cytokine signaling 1
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 1
• NCBI Official Symbol: SOCS1
• NCBI Official Synonym Symbols: JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
• NCBI Protein Information: suppressor of cytokine signaling 1
• UniProt Protein Name: Suppressor of cytokine signaling 1
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS1
• UniProt Synonym Gene Names: SSI1; TIP3; SOCS-1; JAB; SSI-1; TIP-3
• UniProt Entry Name: SOCS1_HUMAN

NCBI Description

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008]

Uniprot Description

SOCS1: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway. Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1. Interacts (via SH2 domain and SOCS box) with TRIM8. Interacts with AXL, CUL2 and FGFR3. Interacts with INSR. By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families. Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 16p13.13

Cellular Component: cytoplasm; cytosol

Molecular Function: insulin-like growth factor receptor binding; kinase inhibitor activity; protein binding; protein kinase binding; protein kinase inhibitor activity

Biological Process: cytokine and chemokine mediated signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of JAK-STAT cascade; negative regulation of tyrosine phosphorylation of Stat3 protein; regulation of protein amino acid phosphorylation