Rabbit anti-Human RMP Polyclonal Antibody | anti-RMP antibody

RMP, CT (Unconventional Prefoldin RPB5 Interactor, RNA Polymerase II Subunit 5-mediating Protein, RPB5-mediating Protein, NNX3, C19orf2, URI) (PE)

Cat. Num. AAA6498081

• Gene Names: URI1; RMP; URI; NNX3; C19orf2; PPP1R19
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: RMP; Polyclonal Antibody; CT (Unconventional Prefoldin RPB5 Interactor; RNA Polymerase II Subunit 5-mediating Protein; RPB5-mediating Protein; NNX3; C19orf2; URI) (PE); anti-RMP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RMP.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RMP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:250-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human RMP (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-RMP Pab is used in Western blot to detect RMP in T47D (left) and Jurkat (right) cell line lysates.)

Product Categories/Family for anti-RMP antibody

Antibodies; Enzymes; Polymerase

References

1. Delgermaa, L., et al., Mol. Cell. Biol. 24(19):8556-8566 (2004). 2. Gstaiger, M., et al., Science 302(5648):1208-1212 (2003). 3. Van Leuven, F., et al., Genomics 54(3):511-520 (1998). 4. Dorjsuren, D., et al., Mol. Cell. Biol. 18(12):7546-7555 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 19924161
• NCBI GeneID: 8725
• NCBI Accession #: NP_604431
• NCBI GenBank Nucleotide #: NM_134447
• UniProt Accession #: O94763
• Molecular Weight: 55kDa
• NCBI Official Synonym Full Names: URI1 prefoldin like chaperone
• NCBI Official Symbol: URI1
• NCBI Official Synonym Symbols: RMP; URI; NNX3; C19orf2; PPP1R19
• NCBI Protein Information: unconventional prefoldin RPB5 interactor 1
• UniProt Protein Name: Unconventional prefoldin RPB5 interactor 1
• Protein Family: RNA polymerase II subunit 5-mediating protein
• UniProt Gene Name: URI1
• UniProt Synonym Gene Names: C19orf2; NNX3; PPP1R19; RMP; URI; RPB5-mediating protein
• UniProt Entry Name: RMP_HUMAN

NCBI Description

This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22. [provided by RefSeq, Nov 2011]

Uniprot Description

RMP: Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein. Homodimer. Component of the URI complex that contains PFDN2, POLR2E/RPB5, RUVBL2, RUVBL1 and URI1. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occurs in a growth-dependent manner in the mitochondrion. Interacts (via the middle C-terminus region) with GTF2F1 and GTF2F2. Interacts with DMAP1, POLR2E/RPB5 and UXT. Ubiquitous. Expressed in ovarian cancers. Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells. Belongs to the RNA polymerase II subunit 5-mediating protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: mitochondrion; dendrite; cytoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: phosphatase inhibitor activity; protein binding; chromatin binding; protein phosphatase inhibitor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; response to virus; regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter