Rabbit CtIP Polyclonal Antibody | anti-CtIP antibody

Anti-Phospho-Ser326 CtIP Antibody

Cat. Num. AAA500067

• Gene Names: RBBP8; RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• Reactivity: Non-Human Primate
• Applications: Western Blot
• Purity: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Synonyms: CtIP; Polyclonal Antibody; Anti-Phospho-Ser326 CtIP Antibody; anti-CtIP antibody

• Host: Rabbit
• Reactivity: Non-Human Primate
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~100 kDa CtIP protein phosphorylated at Ser326. Immunolabeling is completely eliminated by treatment with lambda-Ptase.
• Purity/Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Form/Format: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 897

• Applicable Applications for anti-CtIP antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen Species: Human; Immunogen Information
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser326 of human CtIP.
• Preparation and Storage: Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol.; Stable for at least 1 year at -20 degree C.

Western Blot (WB)

(Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Ser326 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).)

Related Product Information for anti-CtIP antibody

CtIP, C-terminal binding protein-interacting protein, is a DNA endonuclease activated by double stranded breaks (DSBs). DSB repairs can be performed by either one of two mechanisms; non-homologous end joining (NHEJ) or homologous recombination (HR). NHEJ is the predominant DSB repair pathway throughout the entire cell cycle, most importantly in the G1 phase (Rothkamm et al, 2003); while HR is important for repairing DSBs in S and G2 phases (Beucher et al, 2009). CtIP controls DSB resection; an event that only occurs in HR during G2-phase. Phosphorylation of Thr847 dictates the resection efficiency (Huertas et al, 2008). Furthermore, it has been found that DSBs undergo resection and repair in G1-phase cells via a process requiring Plk3 phosphorylation of CtIP at Ser327 and Thr847 (Barton et al, 2014). Several additional phosphorylation sites within CtIP have been identified, but their significance in the repair of DNA have yet to be determined.

References

Rothkamm K, Kruger I, Thompson LH, Lobrich M (2003) Pathways of DNA double-strand break repair during the mammalian cell cycle. Mol Cell Biol., 23, 5706-5715.
Beucher A, Birraux J, tchouandong L, Baron O, Shibata A, Conrad S, Goodarzi AA, Krempler A, Jeggo PA, Lobrich M (2009) ATM and Artemis promote homologous recombination of radiation-inducted DNA double-strand breaks in G2. EMBO J., 28, 3413-3427.
Huertas P, Jackson SP (2008) Human CtIP Mediates Cell Cycle Control of DNA End Resection and Double Strand Break Repair. J. Biol. Chem, 284: 9558-9565.
Olivia Barton, Steffen C Naumann, Ronja Diemer-Biehs, Julia Künzel, Monika Steinlage, Sandro Conrad, Nodar Makharashili, Jiadong Wang, Lin Fent,Bernard S. Lopez, Tanya T. Paull, Junjie Chen, Penny A Jeggo, and Markus Löbrich (2014) Polo-like Kinase 3 regulates CtIP during DNA double-strand break repair in G1. JCB, Nov 7;206(7): 877-894.

NCBI and Uniprot Product Information

• NCBI GI #: 4506441
• NCBI GeneID: 5932
• NCBI Accession #: NP_002885.1
• NCBI GenBank Nucleotide #: NM_002894.2
• UniProt Accession #: Q99708; O75371; Q8NHQ3; A6NKN2; A8K8W6; E7ETY1
• Molecular Weight: ~100 kDa
• NCBI Official Full Name: DNA endonuclease RBBP8 isoform a
• NCBI Official Synonym Full Names: RB binding protein 8, endonuclease
• NCBI Official Symbol: RBBP8
• NCBI Official Synonym Symbols: RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• NCBI Protein Information: DNA endonuclease RBBP8
• UniProt Protein Name: DNA endonuclease RBBP8
• UniProt Gene Name: RBBP8
• UniProt Synonym Gene Names: CTIP; CtIP; RBBP-8; RIM; SAE2

NCBI Description

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:10764811, PubMed:10910365, PubMed:15485915, PubMed:16581787, PubMed:16818604, PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:20829486). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations ().