Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit | SIRPg elisa kit

Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit

Cat. Num. AAA455959

• Gene Names: SIRPG; CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• Reactivity: Human
• Synonyms: Signal Regulatory Protein Gamma (SIRPg); Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit; SIRPg elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SIRPG. No significant cross-reactivity or interference between SIRPG and analogues was observed.
• Sequence Length: 276

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SIRPG were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SIRPG were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIRPg elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SIRPG in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRPG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SIRPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SIRPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SIRPG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 94538339
• NCBI GeneID: 55423
• NCBI Accession #: NP_001034597.1
• NCBI GenBank Nucleotide #: NM_001039508.1
• UniProt Accession #: Q9P1W8; Q5D051; Q5JV25; Q5MKL4; Q8WWA5; Q9NQK8; B1AKP6
• Molecular Weight: 29,967 Da
• NCBI Official Full Name: signal-regulatory protein gamma isoform 3
• NCBI Official Synonym Full Names: signal regulatory protein gamma
• NCBI Official Symbol: SIRPG
• NCBI Official Synonym Symbols: CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• NCBI Protein Information: signal-regulatory protein gamma
• UniProt Protein Name: Signal-regulatory protein gamma
• Protein Family: Signal-regulatory protein
• UniProt Gene Name: SIRPG
• UniProt Synonym Gene Names: SIRPB2; SIRP-gamma; SIRP-b2; SIRP-beta-2

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.