Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit | SIRPg elisa kit

Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit

Cat. Num. AAA2022423

• Gene Names: SIRPG; CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• Reactivity: Human
• Synonyms: Signal Regulatory Protein Gamma (SIRPg); Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit; CD172g; CD172-G; SIRP-G; SIRP-B2; SIRPB2; SIRP-b2; SIRP-beta-2; SIRPgamma; CD172 antigen-like family member B; Signal-regulatory protein beta-2; SIRPg elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Signal Regulatory Protein Gamma (SIRPg).; No significant cross-reactivity or interference between Signal Regulatory Protein Gamma (SIRPg) and analogues was observed.
• Sequence Length: 276

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.067ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Regulatory Protein Gamma (SIRPg) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Regulatory Protein Gamma (SIRPg) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIRPg elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Gamma (SIRPg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Gamma (SIRPg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Gamma (SIRPg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Regulatory Protein Gamma (SIRPg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SIRPg elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 114325406
• NCBI GeneID: 55423
• UniProt Accession #: Q9P1W8; Q5D051; Q5JV25; Q5MKL4; Q8WWA5; Q9NQK8; B1AKP6
• Molecular Weight: 29,967 Da
• NCBI Official Full Name: Signal-regulatory protein gamma
• NCBI Official Synonym Full Names: signal-regulatory protein gamma
• NCBI Official Symbol: SIRPG
• NCBI Official Synonym Symbols: CD172g; SIRPB2; SIRP-B2; bA77C3.1; SIRPgamma
• NCBI Protein Information: signal-regulatory protein gamma
• UniProt Protein Name: Signal-regulatory protein gamma
• Protein Family: Signal-regulatory protein
• UniProt Gene Name: SIRPG
• UniProt Synonym Gene Names: SIRPB2; SIRP-gamma; SIRP-b2; SIRP-beta-2
• UniProt Entry Name: SIRPG_HUMAN

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRPG: Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T- cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; blood coagulation; cell adhesion; leukocyte migration