C1QB recombinant protein

C1QB Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120593

• Purity: >94% as determined by SDS-PAGE
• Synonyms: C1QB; C1QB Protein; Human; Recombinant (His Tag); Human C1QB/C1qB Protein (His Tag); complement component 1; q subcomponent; B chain; C1QB recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, 0.5mM TCEP, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala253

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human C1QB (NP_000482.3) precursor (Met 1-Ala 253) was expressed, with a carboxy-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for C1QB recombinant protein

Background: Complement Component 1, q subcomponent (C1q) associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Southern blot analysis of chromosomal DNA from vertebrate species demonstrated highest similarity between the C1qB genes, followed by C1qC and finally C1qA. Sequence comparison of C1q from three different species have shown that the B chains have the strongest similarity. C1q was already present at embryonic day 14 (E14) and showed little change in abundance through six weeks postnatal. At E16, C1qB mRNA was present at high abundance in putative microglia/macrophages in cortical marginal and intermediate zones, and hippocampal analge.

Product Categories/Family for C1QB recombinant protein

Complement Activation Pathways

References

Pasinetti GM, et al. (1992) Complement C1qB and C4 mRNAs responses to lesioning in rat brain. Experimental neurology. 118(2): 117-25.Johnson SA, et al. (1994) Expression of complement C1qB and C4 mRNAs during rat brain development. Brain Res Dev Brain Res. 80(1-2): 163-74.Grewal RP,et al. (1999) C1qB and clusterin mRNA increase in association with neurodegeneration in sporadic amyotrophic lateral sclerosis. Neuroscience letters. 271(1): 65-7.Spielman L, et al. (2002) Induction of the complement component C1qB in brain of transgenic mice with neuronal overexpression of human cyclooxygenase-2. Acta neuropathologica. 103(2): 157-62.

NCBI and Uniprot Product Information

• NCBI GI #: 87298828
• NCBI GeneID: 713
• NCBI Accession #: NP_000482.3
• NCBI GenBank Nucleotide #: NM_000491.3
• UniProt Accession #: P02746; Q5T959; Q96H17
• Molecular Weight: 26,722 Da
• NCBI Official Full Name: complement C1q subcomponent subunit B
• NCBI Official Synonym Full Names: complement component 1, q subcomponent, B chain
• NCBI Official Symbol: C1QB
• NCBI Protein Information: complement C1q subcomponent subunit B; complement component C1q, B chain; complement subcomponent C1q chain B; complement component 1, q subcomponent, beta polypeptide
• UniProt Protein Name: Complement C1q subcomponent subunit B
• Protein Family: Complement C1q subcomponent
• UniProt Gene Name: C1QB
• UniProt Entry Name: C1QB_HUMAN

NCBI Description

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the B-chain polypeptide of human complement subcomponent C1q [provided by RefSeq, Jul 2008]

Uniprot Description

C1QB: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. Defects in C1QB are a cause of complement component C1q deficiency (C1QD). A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: collagen; extracellular region; complement component C1 complex

Molecular Function: protein binding; protein homodimerization activity

Biological Process: innate immune response; inner ear development; complement activation, classical pathway; complement activation

Disease: C1q Deficiency