Human Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) ELISA Kit | SIGLEC7 elisa kit

Human Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) ELISA Kit

Cat. Num. AAA2703697

• Gene Names: SIGLEC7; p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
• Reactivity: Human
• Synonyms: Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7); Human Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) ELISA Kit; CDw328; CD328; p75; AIRM1; D-Siglec; QA79; SIGLEC-7; P75/AIRM1; Adhesion inhibitory receptor molecule 1; QA79 membrane protein; SIGLEC7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7).; No significant cross-reactivity or interference between Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) and analogues was observed.
• Sequence Length: 467

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIGLEC7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7657570
• NCBI GeneID: 27036
• NCBI Accession #: NP_055200.1
• NCBI GenBank Nucleotide #: NM_014385.3
• UniProt Accession #: Q9Y286; Q9NZQ1; Q9UJ86; Q9UJ87; Q9Y502
• Molecular Weight: 16,740 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 7 isoform 1
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 7
• NCBI Official Symbol: SIGLEC7
• NCBI Official Synonym Symbols: p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
• NCBI Protein Information: sialic acid-binding Ig-like lectin 7
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 7
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC7
• UniProt Synonym Gene Names: AIRM1; Siglec-7; AIRM-1

Uniprot Description

SIGLEC7: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: integral component of plasma membrane; plasma membrane

Molecular Function: carbohydrate binding; receptor activity

Biological Process: cell adhesion; regulation of immune response