Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein phosphatase PGAM5, mitochondrial (PGAM5) Standard Curve.)
Human Serine/Threonine-Protein Phosphatase PGAM5, Mitochondrial (PGAM5) ELISA Kit | PGAM5 elisa kit
Human Serine/Threonine-Protein Phosphatase PGAM5, Mitochondrial (PGAM5) ELISA Kit
Gene Names
PGAM5; BXLBV68
Reactivity
Human
Synonyms
Serine/Threonine-Protein Phosphatase PGAM5; Mitochondrial (PGAM5); Human Serine/Threonine-Protein Phosphatase PGAM5; Mitochondrial (PGAM5) ELISA Kit; PGAM5; BXLBV68; MGC5352; Bcl-XL-binding protein v68; serine/threonine-protein phosphatase PGAM5; mitochondrial; PGAM5 elisa kit
Reactivity
Human
Specificity
Human Serine/threonine-protein phosphatase PGAM5, mitochondrial (PGAM5) ELISA Kit has high sensitivity and excellent specificity for detection of Human PGAM5. No significant cross-reactivity or interference between Human PGAM5 and analogues was observed.
Sequence Length
289
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein phosphatase PGAM5, mitochondrial (PGAM5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein phosphatase PGAM5, mitochondrial (PGAM5) Standard Curve.)
Related Product Information for PGAM5 elisa kit
Description: This Human Serine/threonine-protein phosphatase PGAM5, mitochondrial (PGAM5) ELISA Kit employs a two-site sandwich ELISA to quantitate PGAM5 in samples. An antibody specific for PGAM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGAM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGAM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGAM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Keap1 is a BTB-Kelch substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex that functions as a sensor for thiol-reactive chemopreventive compounds and oxidative stress. PGAM5, as a novel substrate for Keap1. The N terminus of the PGAM5 protein contains a conserved NXESGE motif that binds to the substrate binding pocket in the Kelch domain of Keap1, whereas the C-terminal PGAM domain binds Bcl-XL. Keap1-dependent ubiquitination of PGAM5 results in proteasome-dependent degradation of PGAM5. Quinone-induced oxidative stress and the chemopreventive agent sulforaphane inhibit Keap1-dependent ubiquitination of PGAM5. The identification of PGAM5 as a novel substrate of Keap1 suggests that Keap1 regulates both transcriptional and post-transcriptional responses of mammalian cells to oxidative stress.
Background: Keap1 is a BTB-Kelch substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex that functions as a sensor for thiol-reactive chemopreventive compounds and oxidative stress. PGAM5, as a novel substrate for Keap1. The N terminus of the PGAM5 protein contains a conserved NXESGE motif that binds to the substrate binding pocket in the Kelch domain of Keap1, whereas the C-terminal PGAM domain binds Bcl-XL. Keap1-dependent ubiquitination of PGAM5 results in proteasome-dependent degradation of PGAM5. Quinone-induced oxidative stress and the chemopreventive agent sulforaphane inhibit Keap1-dependent ubiquitination of PGAM5. The identification of PGAM5 as a novel substrate of Keap1 suggests that Keap1 regulates both transcriptional and post-transcriptional responses of mammalian cells to oxidative stress.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine/threonine-protein phosphatase PGAM5, mitochondrial isoform 1
NCBI Official Synonym Full Names
PGAM family member 5, mitochondrial serine/threonine protein phosphatase
NCBI Official Symbol
PGAM5
NCBI Official Synonym Symbols
BXLBV68
NCBI Protein Information
serine/threonine-protein phosphatase PGAM5, mitochondrial
UniProt Protein Name
Serine/threonine-protein phosphatase PGAM5, mitochondrial
Protein Family
UniProt Gene Name
PGAM5
UniProt Entry Name
PGAM5_HUMAN
Research Articles on PGAM5
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Product Notes
The Human PGAM5 pgam5 (Catalog #AAA9714902) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714902 ELISA Kit recognizes Human PGAM5. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Phosphatase PGAM5, Mitochondrial (PGAM5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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