Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 2ug/ml Rabbit Anti-Rat PLCb2 Antibody Second Ab: 0.2ug/mL HRP-LinkedCaprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047
))
Rabbit Phospholipase C Beta 2 Polyclonal Antibody | anti-PLCb2 antibody
Polyclonal Antibody to Phospholipase C Beta 2 (PLCb2)
Immunohistochemistry: 5-20ug/mL; 1:50-200
Immunocytochemistry: 5-20ug/mL; 1:50-200
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 2ug/ml Rabbit Anti-Rat PLCb2 Antibody Second Ab: 0.2ug/mL HRP-LinkedCaprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))
Western Blot (WB)
(Western Blot: Sample: Rat Serum; Primary Ab: 2ug/ml Rabbit Anti-Rat PLCb2 Antibody Second Ab: 0.2ug/mL HRP-LinkedCaprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047
))
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant PLCb2, Rat.)
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant PLCb2, Rat.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
may play a role in inositol mediated signal transduction of bitter taste perception [RGD, Feb 2006]
Uniprot Description
PLCB2: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; Phospholipase
Chromosomal Location of Human Ortholog: 3q35
Cellular Component: cytoplasm; cytosol; Golgi apparatus; plasma membrane
Molecular Function: calcium ion binding; G-protein beta/gamma-subunit complex binding; phosphoinositide phospholipase C activity; phospholipase C activity; phospholipid binding; signal transducer activity
Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste; lipid catabolic process; phospholipid catabolic process; sensory perception of bitter taste
Research Articles on PLCb2
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Product Notes
The PLCb2 plcb2 (Catalog #AAA2033965) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phospholipase C Beta 2 (PLCb2) reacts with Rattus norvegicus (Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase C Beta 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL;1:500-2000 Immunohistochemistry: 5-20ug/mL; 1:50-200 Immunocytochemistry: 5-20ug/mL; 1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PLCb2 plcb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholipase C Beta 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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