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Western Blot (WB) (ATG9B Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the ATG9B antibody detected the ATG9B protein (arrow).)

Rabbit anti-Human ATG9B Polyclonal Antibody | anti-ATG9B antibody

ATG9B, CT (ATG9B, APG9L2, NOS3AS, Autophagy-related protein 9B, APG9-like 2, Nitric oxide synthase 3-overlapping antisense gene protein) (AP)

Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ATG9B; Polyclonal Antibody; CT (ATG9B; APG9L2; NOS3AS; Autophagy-related protein 9B; APG9-like 2; Nitric oxide synthase 3-overlapping antisense gene protein) (AP); Nitric oxide synthase 3-overlapping antisense gene protein; anti-ATG9B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ATG9B antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ATG9B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 891-922 amino acids from the C-terminal region of human ATG9B.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ATG9B Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the ATG9B antibody detected the ATG9B protein (arrow).)

Western Blot (WB) (ATG9B Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the ATG9B antibody detected the ATG9B protein (arrow).)

Immunohistochemistry (IHC)

(ATG9B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATG9B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ATG9B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATG9B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-ATG9B antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Han, S., et al. Hum. Immunol. 71(7):727-730(2010). Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010). Webber, J.L., et al. FEBS Lett. 584(7):1319-1326(2010). Ban, H.J., et al. BMC Genet. 11, 26 (2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,989 Da
NCBI Official Full Name
autophagy-related protein 9B
NCBI Official Synonym Full Names
autophagy related 9B
NCBI Official Symbol
ATG9B
NCBI Official Synonym Symbols
SONE; APG9L2; NOS3AS
NCBI Protein Information
autophagy-related protein 9B; APG9-like 2; ATG9 autophagy related 9 homolog B; autophagy 9-like 2 protein; endothelial nitric oxide synthase antisense; nitric oxide synthase 3-overlapping antisense gene protein
UniProt Protein Name
Autophagy-related protein 9B
Protein Family
UniProt Gene Name
ATG9B
UniProt Synonym Gene Names
APG9L2; NOS3AS; Protein sONE
UniProt Entry Name
ATG9B_HUMAN

NCBI Description

This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]

Uniprot Description

ATG9B: Plays a role in autophagy. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Vesicle; Autophagy

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: integral to membrane; cytoplasmic vesicle

Biological Process: autophagic vacuole formation

Research Articles on ATG9B

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Product Notes

The ATG9B atg9b (Catalog #AAA6276293) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG9B, CT (ATG9B, APG9L2, NOS3AS, Autophagy-related protein 9B, APG9-like 2, Nitric oxide synthase 3-overlapping antisense gene protein) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATG9B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ATG9B atg9b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG9B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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