Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase 31 (STK31) Standard Curve.)
Human Serine/Threonine-Protein Kinase 31 (STK31) ELISA Kit | STK31 elisa kit
Human Serine/Threonine-Protein Kinase 31 (STK31) ELISA Kit
Gene Names
STK31; TDRD8; SGK396
Reactivity
Human
Synonyms
Serine/Threonine-Protein Kinase 31 (STK31); Human Serine/Threonine-Protein Kinase 31 (STK31) ELISA Kit; STK31; FLJ16102; SGK396; TDRD8; OTTHUMP00000201935; sugen kinase 396; tudor domain containing 8; STK31 elisa kit
Reactivity
Human
Specificity
Human Serine/threonine-protein kinase 31 (STK31) ELISA Kit has high sensitivity and excellent specificity for detection of Human STK31. No significant cross-reactivity or interference between Human STK31 and analogues was observed.
Sequence Length
1019
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase 31 (STK31) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase 31 (STK31) Standard Curve.)
Related Product Information for STK31 elisa kit
Description: This Human Serine/threonine-protein kinase 31 (STK31) ELISA Kit employs a two-site sandwich ELISA to quantitate STK31 in samples. An antibody specific for STK31 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STK31 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STK31 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STK31 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: This au protein kinase belongs to the family of transferases, specifically those transferring a phosphate group to the sidechain oxygen atom of serine or threonine residues in proteins.This enzyme participates in 14 metabolic pathways: erbb signaling pathway, cell cycle, wnt signaling pathway, hedgehog signaling pathway, axon guidance, focal adhesion, b cell receptor signaling pathway, insulin signaling pathway, melanogenesis, alzheimer's disease, colorectal cancer, endometrial cancer, prostate cancer, and basal cell carcinoma.Expressed in the brain, particularly in cortical and hippocampal neurons. Weakly expressed in spinal cord and testis. No expression in adipose tissue, bladder, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus, thyroid or trachea.
Background: This au protein kinase belongs to the family of transferases, specifically those transferring a phosphate group to the sidechain oxygen atom of serine or threonine residues in proteins.This enzyme participates in 14 metabolic pathways: erbb signaling pathway, cell cycle, wnt signaling pathway, hedgehog signaling pathway, axon guidance, focal adhesion, b cell receptor signaling pathway, insulin signaling pathway, melanogenesis, alzheimer's disease, colorectal cancer, endometrial cancer, prostate cancer, and basal cell carcinoma.Expressed in the brain, particularly in cortical and hippocampal neurons. Weakly expressed in spinal cord and testis. No expression in adipose tissue, bladder, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus, thyroid or trachea.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine/threonine-protein kinase 31 isoform a
NCBI Official Synonym Full Names
serine/threonine kinase 31
NCBI Official Symbol
STK31
NCBI Official Synonym Symbols
TDRD8; SGK396
NCBI Protein Information
serine/threonine-protein kinase 31
UniProt Protein Name
Serine/threonine-protein kinase 31
Protein Family
UniProt Gene Name
STK31
UniProt Synonym Gene Names
SGK396; SgK396
UniProt Entry Name
STK31_HUMAN
NCBI Description
This gene is similar to a mouse gene that encodes a putative protein kinase with a tudor domain, and shows testis-specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Tissue specificity: Testis specific.
Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 protein kinase domain.Contains 1 Tudor domain.
Caution: Ser-854 is present instead of the conserved Asp which is expected to be an active site residue.
Research Articles on STK31
Similar Products
Product Notes
The Human STK31 stk31 (Catalog #AAA9714055) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714055 ELISA Kit recognizes Human STK31. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Kinase 31 (STK31), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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